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Literature summary for 2.4.1.115 extracted from

  • Hiromoto, T.; Honjo, E.; Noda, N.; Tamada, T.; Kazuma, K.; Suzuki, M.; Blaber, M.; Kuroki, R.
    Structural basis for acceptor-substrate recognition of UDP-glucose: anthocyanidin 3-O-glucosyltransferase from Clitoria ternatea (2015), Protein Sci., 24, 395-407.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
purified enzyme alone and in complex with anthocyanidin delphinidin and petunidin, and flavonol kaempferol, X-ray diffraction structure determination and analysis at 1.85 A, 2.55 A, 2.70 A, and 1.75 A resolution, respectively Clitoria ternatea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Clitoria ternatea the enzyme catalyzes glucosyl transfer from UDP-glucose to anthocyanidins such as delphinidin ?
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?
UDP-D-glucose + delphinidin Clitoria ternatea
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UDP + delphinidin-3-O-beta-D-glucoside
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?
UDP-D-glucose + petunidin Clitoria ternatea
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UDP + petunidin-3-O-beta-D-glucoside
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?

Organism

Organism UniProt Comment Textmining
Clitoria ternatea A4F1R4 gene Ct3GT-A
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme catalyzes glucosyl transfer from UDP-glucose to anthocyanidins such as delphinidin Clitoria ternatea ?
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?
additional information the anthocyanidin- and flavonol-acceptor binding details are almost identical in the enzyme complex structures with anthocyanidin delphinidin and petunidin, and flavonol kaempferol, although the glucosylation activities against each acceptor are significantly different. The 3-hydroxyl groups of the acceptor substrates were located at hydrogen-bonding distances to the Nepsilon2 atom of the His17 catalytic residue, supporting a role for glucosyl transfer to the 3-hydroxyl groups of anthocyanidins and flavonols, substrate recognition, overview. The enzyme shows only low activity with flavonols, i.e. isorhamnetin, quercetin, kaempferol, and myricetin, cf. EC 2.4.1.91 Clitoria ternatea ?
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?
UDP-D-glucose + cyanidin 17% of the activity with delphinidin Clitoria ternatea UDP + cyanidin-3-O-beta-D-glucoside
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?
UDP-D-glucose + delphinidin
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Clitoria ternatea UDP + delphinidin-3-O-beta-D-glucoside
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?
UDP-D-glucose + delphinidin best substrate Clitoria ternatea UDP + delphinidin-3-O-beta-D-glucoside
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?
UDP-D-glucose + malvidin 87% of the activity with delphinidin Clitoria ternatea UDP + malvidin-3-O-beta-D-glucoside
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?
UDP-D-glucose + pelargonidin 29% of the activity with delphinidin Clitoria ternatea UDP + pelargonidin-3-O-beta-D-glucoside
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?
UDP-D-glucose + peonidin 77% of the activity with delphinidin Clitoria ternatea UDP + peonidin-3-O-beta-D-glucoside
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?
UDP-D-glucose + petunidin
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Clitoria ternatea UDP + petunidin-3-O-beta-D-glucoside
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?
UDP-D-glucose + petunidin 72% of the activity with delphinidin Clitoria ternatea UDP + petunidin-3-O-beta-D-glucoside
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?

Synonyms

Synonyms Comment Organism
UDP-glucose: anthocyanidin 3-O-glucosyltransferase
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Clitoria ternatea
UGT78K6
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Clitoria ternatea