Crystallization (Comment) | Organism |
---|---|
purified enzyme alone and in complex with anthocyanidin delphinidin and petunidin, and flavonol kaempferol, X-ray diffraction structure determination and analysis at 1.85 A, 2.55 A, 2.70 A, and 1.75 A resolution, respectively | Clitoria ternatea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Clitoria ternatea | the enzyme catalyzes glucosyl transfer from UDP-glucose to anthocyanidins such as delphinidin | ? | - |
? | |
UDP-D-glucose + delphinidin | Clitoria ternatea | - |
UDP + delphinidin-3-O-beta-D-glucoside | - |
? | |
UDP-D-glucose + petunidin | Clitoria ternatea | - |
UDP + petunidin-3-O-beta-D-glucoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clitoria ternatea | A4F1R4 | gene Ct3GT-A | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme catalyzes glucosyl transfer from UDP-glucose to anthocyanidins such as delphinidin | Clitoria ternatea | ? | - |
? | |
additional information | the anthocyanidin- and flavonol-acceptor binding details are almost identical in the enzyme complex structures with anthocyanidin delphinidin and petunidin, and flavonol kaempferol, although the glucosylation activities against each acceptor are significantly different. The 3-hydroxyl groups of the acceptor substrates were located at hydrogen-bonding distances to the Nepsilon2 atom of the His17 catalytic residue, supporting a role for glucosyl transfer to the 3-hydroxyl groups of anthocyanidins and flavonols, substrate recognition, overview. The enzyme shows only low activity with flavonols, i.e. isorhamnetin, quercetin, kaempferol, and myricetin, cf. EC 2.4.1.91 | Clitoria ternatea | ? | - |
? | |
UDP-D-glucose + cyanidin | 17% of the activity with delphinidin | Clitoria ternatea | UDP + cyanidin-3-O-beta-D-glucoside | - |
? | |
UDP-D-glucose + delphinidin | - |
Clitoria ternatea | UDP + delphinidin-3-O-beta-D-glucoside | - |
? | |
UDP-D-glucose + delphinidin | best substrate | Clitoria ternatea | UDP + delphinidin-3-O-beta-D-glucoside | - |
? | |
UDP-D-glucose + malvidin | 87% of the activity with delphinidin | Clitoria ternatea | UDP + malvidin-3-O-beta-D-glucoside | - |
? | |
UDP-D-glucose + pelargonidin | 29% of the activity with delphinidin | Clitoria ternatea | UDP + pelargonidin-3-O-beta-D-glucoside | - |
? | |
UDP-D-glucose + peonidin | 77% of the activity with delphinidin | Clitoria ternatea | UDP + peonidin-3-O-beta-D-glucoside | - |
? | |
UDP-D-glucose + petunidin | - |
Clitoria ternatea | UDP + petunidin-3-O-beta-D-glucoside | - |
? | |
UDP-D-glucose + petunidin | 72% of the activity with delphinidin | Clitoria ternatea | UDP + petunidin-3-O-beta-D-glucoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucose: anthocyanidin 3-O-glucosyltransferase | - |
Clitoria ternatea |
UGT78K6 | - |
Clitoria ternatea |