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Literature summary for 2.4.1.11 extracted from
- Molecular cloning and characterization of glycogen synthase in Eriocheir sinensis (2017), Comp. Biochem. Physiol. B, 214, 47-56 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta (DE3) cells | Eriocheir sinensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n | Eriocheir sinensis | - |
UDP + [(1->4)-alpha-D-glucosyl]n+1 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Eriocheir sinensis | - |
Chinese mitten crab | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Hi Trap column chromatography and Sepharose 6 column chromatography | Eriocheir sinensis |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| using 50 mM Tris, 5 mM L(+)-cysteine, 8-1 M urea, pH 7.4 at 4°C for 4-6 h | Eriocheir sinensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Eriocheir sinensis | - |
| gill | - |
Eriocheir sinensis | - |
| gut | - |
Eriocheir sinensis | - |
| heart | - |
Eriocheir sinensis | - |
| hepatopancreas | highest expression | Eriocheir sinensis | - |
| muscle | - |
Eriocheir sinensis | - |
| ovary | - |
Eriocheir sinensis | - |
| stomach | - |
Eriocheir sinensis | - |
| testis | - |
Eriocheir sinensis | - |
| thoracic ganglion | - |
Eriocheir sinensis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n | - |
Eriocheir sinensis | UDP + [(1->4)-alpha-D-glucosyl]n+1 | - |
? | |
| UDP-glucose + glycogen | - |
Eriocheir sinensis | glycogen + UDP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 79000, calculated from amino acid sequence | Eriocheir sinensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glycogen synthase | - |
Eriocheir sinensis |
| GS-I | - |
Eriocheir sinensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Eriocheir sinensis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Eriocheir sinensis | calculated from amino acid sequence | - |
5.9 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Eriocheir sinensis | enzyme expression and activity decline during moulting | down |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is important for maintaining normal physiological functions such as growth and reproduction | Eriocheir sinensis |
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