Cloned (Comment) | Organism |
---|---|
PCR-amplification of reference strain open reading frames cloned into standard cloning vectors, expressed in fungus | Cryptococcus neoformans var. neoformans |
PCR-amplification of reference strain open reading frames cloned into standard cloning vectors, expressed in fungus | Cryptococcus neoformans var. grubii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | disruption after amino acid D439 results in pmt1A lacking mutants are viable but show distinct defects in cell morphology and cell integrity (wild-type comparable growth at 30°C, reduced growth at 37°C, no growth at 39°C in contrast to wild-type, more susceptible to SDS medium, enlarged cells subject to spontaneous lysis, attenuated virulence), Pmt1/Pmt4 double mutants are not viable, pmt1A mutant is susceptible to sorbitol (2.5 M) and to 0.1% SDS, reduced survival upon macrophage attack | Cryptococcus neoformans var. grubii |
additional information | disruption after amino acid E468 results in pmt2A lacking mutants, not viable, one wild-type allele is necessary for growth, after sporulation no haploid pmt2 lacking mutants are found, Pmt2 is essential but an intact Pmt2 gene lacking the other two isoforms is not sufficient for viability, may be due to interaction with the other isoforms | Cryptococcus neoformans var. grubii |
additional information | disruption after amino acid K461 results in pmt4A lacking mutants, viable but with distinct defects in cell morphology and cell integrity (wild-type comparable growth at 30°C, reduced growth at 37°C, no growth at 39°C in contrast to wild-type, poor growth on high salt medium, abnormal septum formation, enlarged cells subject to spontaneous lysis, multi-cell aggregate formation based on defects in cell separation, delayed melanin production, slightly reduced capsule size, attenuated virulence), Pmt1/Pmt4 double mutants are not viable, points to necessary interaction with the Pmt2 isoform or essential combined target, the pmt4A mutant is susceptible to sorbitol (2 M), reduced survival upon macrophage attack | Cryptococcus neoformans var. grubii |
additional information | disruption after amino acid N508 results in pmt2D lacking mutants, not viable, one wild-type allele is necessary for growth, after sporulation no haploid pmt2 lacking mutants are found, Pmt2 is essential but an intact Pmt2 gene lacking the other two isoforms is not sufficient for viability may be due to interaction with the other isoforms | Cryptococcus neoformans var. neoformans |
additional information | disruption after amino acid Q412 results in pmt4D lacking mutants, aviable but with distinct defects in cell morphology and cell integrity (wild-type comparable growth at 30°C, reduced growth at 37°C, no growth at 39°C in contrast to wild-type, poor growth on high salt medium, abnormal septum formation, enlarged cells subject to spontaneous lysis, multi-cell aggregate formation based on defects in cell separation, delayed melanin production, slightly reduced capsule size, attenuated virulence), Pmt1/Pmt4 double mutants are not viable, points to necessary interaction with the Pmt2 isoform or essential combined target, the pmt4D strain is not inhibited by sorbitol but is inhibited by SDS, bilateral crossing of two pmt4D mutant strains delays mating reaction with reduced filament formation, less aerial hyphae, irregular shape and thickness of filaments with swollen distal tips | Cryptococcus neoformans var. neoformans |
additional information | disruption after amino acid W315 results in pmt1D lacking mutants are viable but show distinct defects in cell morphology and cell integrity (wild-type comparable growth at 30°C, reduced growth at 37°C, no growth at 39°C in contrast to wild-type, more susceptible to SDS medium, enlarged cells subject to spontaneous lysis, attenuated virulence), Pmt1/Pmt4 double mutants are not viable, the pmt1D mutant grows poorly in sorbitol, is unaffected by SDS, no defects in mating and crossing are observed | Cryptococcus neoformans var. neoformans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | cell wall destabilizing agents Congo red, caffeine, and calcofluor white have no effect on the growth of any pmt mutants | Cryptococcus neoformans var. grubii | |
additional information | cell wall destabilizing agents Congo red, caffeine, and calcofluor white have no effect on the growth of any pmt mutants; cell wall destabilizing agents Congo red, caffeine, and calcofluor white have no effect on the growth of any pmt mutants | Cryptococcus neoformans var. neoformans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Cryptococcus neoformans var. neoformans | 5783 | - |
endoplasmic reticulum | - |
Cryptococcus neoformans var. grubii | 5783 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | at 0.5 M KCl pmt4 mutant grows slower than wild-type, at 0.7 M growth inhibition | Cryptococcus neoformans var. neoformans | |
additional information | at 0.5 M KCl pmt4 mutant grows slower than wild-type, at 0.7 M growth inhibition | Cryptococcus neoformans var. grubii | |
additional information | at 1 M MaCl growth inhibition of mutant pmt1 but to a lesser extent than mutant pmt4 | Cryptococcus neoformans var. grubii | |
additional information | at 1 M NaCl growth inhibition of mutant pmt1 but to a lesser extent than mutant pmt4 | Cryptococcus neoformans var. neoformans | |
additional information | at 1 M NaCl growth inhibition of mutant pmt4 | Cryptococcus neoformans var. neoformans | |
additional information | at 1 M NaCl growth inhibition of mutant pmt4 | Cryptococcus neoformans var. grubii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl phosphate D-mannose + protein | Cryptococcus neoformans var. neoformans | - |
dolichyl phosphate + O-D-mannosylprotein | - |
? | |
dolichyl phosphate D-mannose + protein | Cryptococcus neoformans var. grubii | - |
dolichyl phosphate + O-D-mannosylprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cryptococcus neoformans var. grubii | - |
serotype A, wild-type strain H99, human fungal pathogen, strain used for pathogenesis experiments | - |
Cryptococcus neoformans var. neoformans | Q5KAF1 | serotype D, wild-type strain JEC21 and JEC21, human fungal pathogen, strain used for pathogenesis experiments | - |
Cryptococcus neoformans var. neoformans | Q5KHK5 | serotype D, wild-type strain JEC21 and JEC21, human fungal pathogen, strain used for pathogenesis experiments | - |
Cryptococcus neoformans var. neoformans | Q5KIZ1 | serotype D, wild-type strain JEC21 and JEC21, human fungal pathogen, strain used for pathogenesis experiments | - |
Purification (Comment) | Organism |
---|---|
gel purification of disruption mutants | Cryptococcus neoformans var. neoformans |
gel purification of disruption mutants | Cryptococcus neoformans var. grubii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl phosphate D-mannose + protein | - |
Cryptococcus neoformans var. neoformans | dolichyl phosphate + O-D-mannosylprotein | - |
? | |
dolichyl phosphate D-mannose + protein | - |
Cryptococcus neoformans var. grubii | dolichyl phosphate + O-D-mannosylprotein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Pmt1A | - |
Cryptococcus neoformans var. grubii |
Pmt1D | - |
Cryptococcus neoformans var. neoformans |
Pmt2A | - |
Cryptococcus neoformans var. grubii |
Pmt2D | - |
Cryptococcus neoformans var. neoformans |
Pmt4A | - |
Cryptococcus neoformans var. grubii |
Pmt4D | - |
Cryptococcus neoformans var. neoformans |
protein O-mannosyltransferase | - |
Cryptococcus neoformans var. neoformans |
protein O-mannosyltransferase | - |
Cryptococcus neoformans var. grubii |
Organism | Comment | Expression |
---|---|---|
Cryptococcus neoformans var. neoformans | no change in expression of either isoform upon growth in nutrient rich medium at 30 and 37°C, with additional salt stress or under capsule-inducing conditions, expression is not unregulated due to deficiency in one other isoform | additional information |
Cryptococcus neoformans var. grubii | no change in expression of either isoform upon growth in nutrient rich medium at 30 and 37°C, with additional salt stress or under capsule-inducing conditions, expression is not unregulated due to deficiency in one other isoform | additional information |
General Information | Comment | Organism |
---|---|---|
malfunction | virulence of the human fungal pathogen causing meningoencephalitis depends on extracellular factors including the O-glycosylation of proteins | Cryptococcus neoformans var. neoformans |
malfunction | virulence of the human fungal pathogen causing meningoencephalitis depends on extracellular factors including the O-glycosylation of proteins | Cryptococcus neoformans var. grubii |
physiological function | O-glycosylation of proteins | Cryptococcus neoformans var. neoformans |
physiological function | O-glycosylation of proteins | Cryptococcus neoformans var. grubii |