General Stability | Organism |
---|---|
stable in solution with deoxycholate and CHAPS | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycerol | at 20% inhibitory | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Saccharomyces cerevisiae | 16020 | - |
microsome | - |
Saccharomyces cerevisiae | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl phosphate D-mannose + protein | Saccharomyces cerevisiae | - |
dolichyl phosphate + O-D-mannosylprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
gene PMT1 | - |
Purification (Comment) | Organism |
---|---|
partial | Saccharomyces cerevisiae |
solubilization with 0.5% deoxycholate and 1.2% CHAPS | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0035 | - |
partially purified enzyme | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-80°C, partially purified enzyme, indefinitely stable | Saccharomyces cerevisiae |
23°C, partially purified enzyme, 2% glycerol, at least 12 h stable | Saccharomyces cerevisiae |
4-23°C, partially purified enzyme, stable for at least 1 week | Saccharomyces cerevisiae |
4°C, solubilized with 0.5% Triton X-100, loss of 80% activity within 5 days | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl phosphate D-mannose + biotin-Tyr-Ala-Thr-Ala-Val-NH2 | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosyl-N-biotinyl-Tyr-Ala-Thr-Ala-Val-NH2 | - |
? | |
dolichyl phosphate D-mannose + protein | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
? | |
dolichyl phosphate D-mannose + protein | the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues | Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
? | |
dolichyl phosphate D-mannose + protein | preferred chain lengthin decreasing order: C100, C80, C55, C35 | Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
? | |
dolichyl phosphate D-mannose + protein | strictly stereospecific for the anomeric configuration of phosphoryl-linkage of the donor substrate, a saturated alpha-isoprene unit in the dolichyl moiety is required | Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
? | |
dolichyl phosphate D-mannose + Tyr-Asn-Pro-Thr-Ser-Val-NH2 | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-alpha-D-mannosyl-Tyr-Asn-Pro-Thr-Ser-Val-NH2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PMT | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.9 | - |
inactivation below pH 4.9 | Saccharomyces cerevisiae |