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Literature summary for 2.4.1.103 extracted from

  • Mateju, J.; Cudlin, J.; Steinerova, N.; Blumauerova, M.; Vanek, Z.
    Partial purification and properties of glucosyltransferase from Streptomyces aureofaciens (1979), Folia Microbiol. (Praha), 24, 205-210.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
alizarin pH 7.4, 30°C Kitasatospora aureofaciens
0.0108
-
UDPglucose pH 7.4, 30°C Kitasatospora aureofaciens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Kitasatospora aureofaciens
-
-

Organism

Organism UniProt Comment Textmining
Kitasatospora aureofaciens
-
mutant strain B96
-

Purification (Commentary)

Purification (Comment) Organism
partial Kitasatospora aureofaciens

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Kitasatospora aureofaciens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Kitasatospora aureofaciens

Storage Stability

Storage Stability Organism
-20°C, 2 months Kitasatospora aureofaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + alizarin i.e.1,2-dihydroxy-9,10-anthraquinone Kitasatospora aureofaciens UDP + 1-hydroxy-2-(beta-D-glucosyloxy)-9,10-anthraquinone
-
?
UDP-glucose + 1,4-dihydroxy-9,10-anthraquinone 7% of the activity with 1,2-dihydroxy-9,10-anthraquinone Kitasatospora aureofaciens UDP + 1-hydroxy-4-(beta-D-glucosyloxy)-9,10-anthraquinone
-
?
UDP-glucose + 1,5-dihydroxy-9,10-anthraquinone 2% of the activity with 1,2-dihydroxy-9,10-anthraquinone Kitasatospora aureofaciens UDP + 1-hydroxy-5-(beta-D-glucosyloxy)-9,10-anthraquinone
-
?
UDPglucose + 1,8-dihydroxy-9,10-anthraquinone 9% of the activity with 1,2-dihydroxy-9,10-anthraquinone Kitasatospora aureofaciens UDP + 1-hydroxy-8-(beta-D-glucosyloxy)-9,10-anthraquinone
-
?
UDPglucose + 2,6-dihydroxy-9,10-anthraquinone 51% of the activity with 1,2-dihydroxy-9,10-anthraquinone Kitasatospora aureofaciens UDP + 1-hydroxy-6-(beta-D-glucosyloxy)-9,10-anthraquinone
-
?
UDPglucose + 1-hydroxy-9,10-anthraquinone 3% of the activity with 1,2-dihydroxy-9,10-anthraquinone Kitasatospora aureofaciens UDP + 1-(beta-D-glucosyloxy)-9,10-anthraquinone
-
?
UDPglucose + 2-hydroxy-9,10-anthraquinone 32% of the activity with 1,2-dihydroxy-9,10-anthraquinone Kitasatospora aureofaciens UDP + 2-(beta-D-glucosyloxy)-9,10-anthraquinone
-
?
UDPglucose + 1,3-dihydroxy-9,10-anthraquinone 48% of the activity with 1,2-dihydroxy-9,10-anthraquinone Kitasatospora aureofaciens UDP + 1-hydroxy-3-(beta-D-glucosyloxy)-9,10-anthraquinone
-
?
UDPglucose + 3-hydroxy-1-methoxy-9,10-anthraquinone 52% of the activity with 1,2-dihydroxy-9,10-anthraquinone Kitasatospora aureofaciens UDP + 1-methoxy-3-(beta-D-glucosyloxy)-9,10-anthraquinone
-
?
UDPglucose + 1-hydroxy-3-methoxy9,10-anthraquinone 2% of the activity with 1,2-dihydroxy-9,10-anthraquinone Kitasatospora aureofaciens UDP + 3-methoxy-1-(beta-D-glucosyloxy)-9,10-anthraquinone
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Kitasatospora aureofaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
glucosylation of 1,2-dihydroxy-9,10-anthraquinone Kitasatospora aureofaciens

pH Range

pH Minimum pH Maximum Comment Organism
6.8 7.6 about half-maximal activity at pH 6.7 and 7.6 Kitasatospora aureofaciens