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Literature summary for 2.4.1.102 extracted from
- Structural and mechanistic characterization of leukocyte-type core 2 ?1,6-N-acetylglucosaminyltransferase: a metal-ion-independent GT-A glycosyltransferase (2011), J. Mol. Biol., 414, 798-811.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mutant enzymes are expressed in HEK-293T cells | Mus musculus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| C217S mutant in complex with UDP, hanging drop vapor diffusion method, using 24% (w/v) polyethylene glycol 4000, 0.1 M glycine (pH 9.0) and 0.6 M LiCl | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C217S | the mutant is catalytically active | Mus musculus |
| K401A/C217S | inactive | Mus musculus |
| R378A/C217S | inactive | Mus musculus |
| R378A/K401A/C217S | inactive | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| UDP | - |
Mus musculus |  |
| UMP | - |
Mus musculus | |
| uridine | - |
Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.8 | - |
UDP-N-acetyl-D-glucosamine | mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C | Mus musculus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q09324 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| IgG-Sepharose bead chromatography | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leukocyte | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine + Galbeta1-3GalNAcalpha-4-nitrophenyl | - |
Mus musculus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| C2GnT-I | - |
Mus musculus |
| C2GnT-L | - |
Mus musculus |
| core 2 beta1,6-N-acetylglucosaminyltransferase | - |
Mus musculus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
UMP | mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C | Mus musculus | |
| 0.26 | - |
UDP | mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C | Mus musculus | |
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Release 2023.1 (January 2023)
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