Literature summary for 2.4.1.101 extracted from

Li, Y.; Liu, Y.; Zhu, H.; Chen, X.; Tian, M.; Wei, Y.; Gong, Y.; Jiang, J.
N-acetylglucosaminyltransferase I promotes glioma cell proliferation and migration through increasing the stability of the glucose transporter GLUT1 (2020), FEBS Lett., 594, 358-366 .

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Protein Variants

Protein Variants	Comment	Organism
additional information	MGAT1 protein and mRNA levels are markedly reduced in cells infected with lentiviral expressing MGAT1 shRNA-1 or MGAT1-shRNA-2. Knockdown of MGAT1 leads to an increase in the G1-phase cells with a decrease in S-phase cells and G2/M-phase cells. Downregulation of MGAT1 significantly inhibits migration of GBM cells	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi membrane	-	Homo sapiens	139	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P26572	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Homo sapiens	-
glioblastoma cell	MGAT1 is highly expressed in glioblastoma tissues	Homo sapiens	-
glioma cell	cell proliferation and migration analysis, overview	Homo sapiens	-
U-251MG cell	-	Homo sapiens	-
U-87MG cell	-	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
MGAT1	-	Homo sapiens
N-acetylglucosaminyltransferase I	-	Homo sapiens

General Information

General Information	Comment	Organism
malfunction	downregulation of MGAT1 inhibits glioma cell proliferation and migration, overview. Glut1 protein expression is significantly decreased in MGAT1 knockdown cells, but there is no significant change in Glut3. Activation of EGFR signalling by HB-EGF can rescue the inhibitory effects of MGAT1 knockdown on the expression of Glut1, but downregulation of MGAT1 does not significantly change the level of Glut1 mRNA. Downregulation of MGAT1 decreases the complex N-glycan of Glut1. Ectopic expression of Glut1 rescues the inhibitory effects of MGAT1 knockdown on glioma cell proliferation and migration	Homo sapiens
additional information	Glut1 N-glycan structure is evaluated by N-glycosidase digestion. Glut1 in U87/MG cells is sensitive to PNGase F but resistant to endo H digestion, suggesting that the type of Glut1 N-glycan in U87/MG cells are mainly complex type N-glycan	Homo sapiens
physiological function	N-acetylglucosaminyltransferase I (MGAT1) is responsible for the conversion of high-mannose to hybrid and complex N-glycans. It promotes glioma cell proliferation and migration through increasing the stability of the glucose transporter GLUT1, MGAT1 interacted with Glut1 in U-87/MG cells, mechanism of MGAT1 promoting glioma cell proliferation, overview. MGAT1 regulates Glut1 N-glycosylation	Homo sapiens

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Release 2023.1 (January 2023)