Cloned (Comment) | Organism |
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transient expression of chimeric enzyme domain mutants in Nicotiana benthamiana | Nicotiana benthamiana |
Protein Variants | Comment | Organism |
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additional information | construction of a series of chimeric N-acetylglucosaminyltransferase I (GnTI) mutants by to investigate the contribution of the cytoplasmic, transmembrane, and stem region the enzyme for its cis/medial-Golgi localization and for protein-protein interaction in the Golgi apparatus. The individual GnTI protein domains, i.e. cytoplasmic tail, transmembrane domain and luminal stem region, are replaced with those from the well-known trans-Golgi enzyme alpha2,6-sialyltransferase and transiently expressed in Nicotiana benthamiana. A chimeric GnTI variant with altered sub-Golgi localization is not able to complement the GnTI-dependent glycosylation defect. Generation of transgenic Arabdiopsis thaliana gntI plants expressing the chimeric CTS domai region fused to the catalytic domain of Arabidopsis thaliana GnTI enzyme and expression in enzyme activity-deficient Nicotiana benthamiana plants under control of the endogenous GnTI promoter. The Arabidopsis enzyme chimeric constructs can complement the inactive the N-glycan processing defect of tobacco mutant plants | Nicotiana benthamiana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
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Golgi membrane | the enzyme is a Golgi-resident type-II membrane protein. The transmembrane domain of enzyme GnTI is the major determinant for its cis/medial-Golgi localization, while the stem region of GnTI contributes predominately to homomeric and heteromeric protein complex formation | Nicotiana benthamiana | 139 | - |
Organism | UniProt | Comment | Textmining |
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Nicotiana benthamiana | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
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leaf | - |
Nicotiana benthamiana | - |
Subunits | Comment | Organism |
---|---|---|
homodimer | the enzyme has cytoplasmic transmembrane and stem regions. The transmembrane domain of enzyme GnTI is the major determinant for its cis/medial-Golgi localization, while the stem region of GnTI contributes predominately to homomeric and heteromeric protein complex formation | Nicotiana benthamiana |
Synonyms | Comment | Organism |
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GnTI | - |
Nicotiana benthamiana |
N-acetylglucosaminyltransferase I | - |
Nicotiana benthamiana |
General Information | Comment | Organism |
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additional information | steady-state distribution of the enzyme in the cis/medial-Golgi in plants is a prerequisite for efficient N-glycan processing in vivo | Nicotiana benthamiana |