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Literature summary for 2.4.1.101 extracted from

  • Schoberer, J.; Vavra, U.; Stadlmann, J.; Hawes, C.; Mach, L.; Steinkellner, H.; Strasser, R.
    Arginine/lysine residues in the cytoplasmic tail promote ER export of plant glycosylation enzymes (2009), Traffic, 10, 101-115.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.101

Cloned(Commentary)

Cloned (Comment) Organism
PCR-amplification of CTS region (N-terminal cytoplasmic tail, transmembrane domain, stem region), fusion to GFP (green fluorescent protein), expression of vector in Nicotiana benthamiana mediated by Agrobacterium tumefaciens strain UIA143 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information AAA, ER steady-state location with little Golgi labelling Arabidopsis thaliana
additional information AAR, similar Golgi-targeting as wild-type Arabidopsis thaliana
additional information RAA, localisation in ER and Golgi membrane, more pronounced labeling in the ER Arabidopsis thaliana
additional information site-directed mutagenesis of single arginine residues within cytoplasmic tail are sufficient to promote rapid Golgi targeting of Golgi-resident N-acetylglucosaminyltransferase I, an intact ER export motif is essential for proper in vivo function, transport is COPII-dependent, exchange of the cytoplasmic tail with the corresponding part of other plant glycosylation enzymes does not alter the Golgi targeting, complementation experiments with cg/1 Arabidopsis thaliana lacking an active N-acetylglucosaminyltransferase I Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane
-
 Arabidopsis thaliana 5789
-
Golgi membrane
-
 Arabidopsis thaliana 139
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 wild-type and cg/1 plants lacking an own active N-acetylglucosaminyltransferase Is
-

Purification (Commentary)

Purification (Comment) Organism
antibody purification from homogenized transfected Nicotiniana benthamiana leaves, resuspended in extraction buffer (PBS, pH 7.2, 1% Triton-X-100, protease inhibitor cocktail), centrifugation, supernatant incubated with rProtein A-Sepharose, elution by boiling Laemmli sample buffer, protein gel blot analysis with specific antibodies Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
GnTI
-
 Arabidopsis thaliana
N-acetylglucosaminyltransferase I
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function plant N-glycan processing Arabidopsis thaliana