Literature summary for 2.4.1.101 extracted from

Unligil, U.M.; Zhou, S.; Yuwaraj, S.; Sarkar, M.; Schachter, H.; Rini, J.M.
X-ray crystal structure of rabbit N-acetylglucosaminyltransferase I: catalytic mechanism and a new protein superfamily (2000), EMBO J., 19, 5269-5280.

Search for more literature for 2.4.1.101

Cloned(Commentary)

Cloned (Comment)	Organism
expression of catalytic fragment in Spodoptera frugiperda Sf9 cells via baculovirus infection	Oryctolagus cuniculus

Crystallization (Commentary)

Crystallization (Comment)	Organism
catalytic enzyme fragment in presence and absence of UDP-N-acetyl-D-glucosamine and Mn2+, hanging drop vapour diffusion method, protein 10 mg/ml + 10 mM MES, pH 5.5, + 270 mM KCl + 2 mM MnCl2, 10 mM UDP-N-acetyl-D-glucosamine, well-solution: 100 mM Tris-HCl, pH 7.9, 15-20% polyethylene glycol , X-ray structure determination and analysis	Oryctolagus cuniculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	binds not independently of UDP-N-acetyl-D-glucose	Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
additional information	-	2 domain protein, determination of acceptor substrate-, nucleotide sugar- and metal-binding sites	Oryctolagus cuniculus

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	P27115	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant catalytic fragment from Sf9 insect cells	Oryctolagus cuniculus

Reaction

Reaction	Comment	Organism	Reaction ID
UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein]	active site	Oryctolagus cuniculus	a
UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein]	ordered sequential bi bi mechanism	Oryctolagus cuniculus	a
UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein]	SGC domain of SGC superfamily	Oryctolagus cuniculus	a
UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein]	DxD motif	Oryctolagus cuniculus	a

Synonyms

Synonyms	Comment	Organism
GnT-I	-	Oryctolagus cuniculus

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Release 2023.1 (January 2023)