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Literature summary for 2.4.1.101 extracted from

  • Nishikawa, Y.; Pegg, W.; Paulsen, H.; Schachter, H.
    Control of glycoprotein synthesis. Purification and characterization of rabbit liver UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I (1988), J. Biol. Chem., 263, 8270-8281.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation by 2-mercaptoethanol Oryctolagus cuniculus
Triton X-100
-
Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
2'-dUDP weak Oryctolagus cuniculus
2'-O-Methyl-UDP weak Oryctolagus cuniculus
5-Bromo-UTP
-
Oryctolagus cuniculus
5-Mercury-UDP
-
Oryctolagus cuniculus
alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
Oryctolagus cuniculus
additional information no inhibitors are CDP, UDPhexanolamine, AMP Oryctolagus cuniculus
TDP
-
Oryctolagus cuniculus
UDP
-
Oryctolagus cuniculus
UDP-glucose weak Oryctolagus cuniculus
UDP-N-acetylgalactosamine weak Oryctolagus cuniculus
UDP-N-acetylglucosamine competitively to UDP Oryctolagus cuniculus
UMP weak Oryctolagus cuniculus
UTP weak Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic study Oryctolagus cuniculus
0.0384
-
UDP-N-acetyl-D-glucosamine
-
Oryctolagus cuniculus
0.0384
-
alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
Oryctolagus cuniculus
2.03
-
alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosamine
-
Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Oryctolagus cuniculus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ slight activation Oryctolagus cuniculus
Ca2+ slight activation Oryctolagus cuniculus
Cd2+ activation Oryctolagus cuniculus
Cd2+ can substitute for Mn2+ with 20% Oryctolagus cuniculus
Co2+ activation Oryctolagus cuniculus
Co2+ can substitute for Mn2+ with 80% Oryctolagus cuniculus
Fe2+ activation Oryctolagus cuniculus
Fe2+ can substitute for Mn2+ with 65% efficiency Oryctolagus cuniculus
Mg2+ activation Oryctolagus cuniculus
Mg2+ can substitute for Mn2+ with 40% Oryctolagus cuniculus
Mn2+ requirement Oryctolagus cuniculus
additional information no activation by Cu2+ Oryctolagus cuniculus
additional information no activation by Sr2+, Pb2+ Oryctolagus cuniculus
Ni2+ activation Oryctolagus cuniculus
Ni2+ can substitute for Mn2+ with 20% Oryctolagus cuniculus
Sn2+ slight activation Oryctolagus cuniculus
Zn2+ activation Oryctolagus cuniculus
Zn2+ can substitute for Mn2+ with 8% Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
separable by gel filtration Oryctolagus cuniculus
additional information
-
two MW-species Oryctolagus cuniculus
45000
-
x * 45000 + x * 50000 + x * 54000, low molecular weight form, SDS-PAGE, major band: MW 45000 Oryctolagus cuniculus
50000
-
x * 45000 + x * 50000 + x * 54000, low molecular weight form, SDS-PAGE, major band: MW 45000 Oryctolagus cuniculus
54000
-
x * 45000 + x * 50000 + x * 54000, low molecular weight form, SDS-PAGE, major band: MW 45000 Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide Oryctolagus cuniculus key enzyme of biosynthesis of complex and hybrid N-glycans UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide
-
?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two molecular weight forms separable by gel filtration, purification of low-molecular weight form Oryctolagus cuniculus

Reaction

Reaction Comment Organism Reaction ID
UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein] mechanism Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.8
-
low-molecular weight form Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information acts on synthetic substrate analogues Oryctolagus cuniculus ?
-
?
additional information no acceptor substrates are N-acetyl-D-glucosamine, N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-1,4-N-acetyl-D-glucosaminyl-Asn Oryctolagus cuniculus ?
-
?
UDP + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosamine
-
Oryctolagus cuniculus ?
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: H Oryctolagus cuniculus UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: H ?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R 1,4-N-acetyl-D-glucosaminyl-Asn Oryctolagus cuniculus UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: H ?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R synthetic-beta-D-mannosyl-(1,6-anhydro)-derivative acts as substrate, too Oryctolagus cuniculus UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: H ?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide key enzyme of biosynthesis of complex and hybrid N-glycans Oryctolagus cuniculus UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R substrate specificity Oryctolagus cuniculus UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: 1,4-N-acetyl-D-glucosaminyl-Asn ?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R essential for activity: unsubstituted equatorial hydroxyl on C-4 of beta-linked mannosyl-residue Oryctolagus cuniculus UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: 1,4-N-acetyl-D-glucosaminyl-Asn ?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: 1,4-N-acetyl-D-glucosaminyl-Asn Oryctolagus cuniculus UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: 1,4-N-acetyl-D-glucosaminyl-Asn ?

Subunits

Subunits Comment Organism
? x * 45000 + x * 50000 + x * 54000, low molecular weight form, SDS-PAGE, major band: MW 45000 Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
Oryctolagus cuniculus

pH Range

pH Minimum pH Maximum Comment Organism
5.1 6.9 about half-maximal activity at pH 5.1 and about 70% of maximal activity at pH 6.9 Oryctolagus cuniculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.034
-
UDP-N-acetylglucosamine
-
Oryctolagus cuniculus
1.8
-
alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
Oryctolagus cuniculus