Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by 2-mercaptoethanol | Oryctolagus cuniculus | |
Triton X-100 | - |
Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-dUDP | weak | Oryctolagus cuniculus | |
2'-O-Methyl-UDP | weak | Oryctolagus cuniculus | |
5-Bromo-UTP | - |
Oryctolagus cuniculus | |
5-Mercury-UDP | - |
Oryctolagus cuniculus | |
alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
Oryctolagus cuniculus | |
additional information | no inhibitors are CDP, UDPhexanolamine, AMP | Oryctolagus cuniculus | |
TDP | - |
Oryctolagus cuniculus | |
UDP | - |
Oryctolagus cuniculus | |
UDP-glucose | weak | Oryctolagus cuniculus | |
UDP-N-acetylgalactosamine | weak | Oryctolagus cuniculus | |
UDP-N-acetylglucosamine | competitively to UDP | Oryctolagus cuniculus | |
UMP | weak | Oryctolagus cuniculus | |
UTP | weak | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study | Oryctolagus cuniculus | |
0.0384 | - |
UDP-N-acetyl-D-glucosamine | - |
Oryctolagus cuniculus | |
0.0384 | - |
alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
Oryctolagus cuniculus | |
2.03 | - |
alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosamine | - |
Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Oryctolagus cuniculus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | slight activation | Oryctolagus cuniculus | |
Ca2+ | slight activation | Oryctolagus cuniculus | |
Cd2+ | activation | Oryctolagus cuniculus | |
Cd2+ | can substitute for Mn2+ with 20% | Oryctolagus cuniculus | |
Co2+ | activation | Oryctolagus cuniculus | |
Co2+ | can substitute for Mn2+ with 80% | Oryctolagus cuniculus | |
Fe2+ | activation | Oryctolagus cuniculus | |
Fe2+ | can substitute for Mn2+ with 65% efficiency | Oryctolagus cuniculus | |
Mg2+ | activation | Oryctolagus cuniculus | |
Mg2+ | can substitute for Mn2+ with 40% | Oryctolagus cuniculus | |
Mn2+ | requirement | Oryctolagus cuniculus | |
additional information | no activation by Cu2+ | Oryctolagus cuniculus | |
additional information | no activation by Sr2+, Pb2+ | Oryctolagus cuniculus | |
Ni2+ | activation | Oryctolagus cuniculus | |
Ni2+ | can substitute for Mn2+ with 20% | Oryctolagus cuniculus | |
Sn2+ | slight activation | Oryctolagus cuniculus | |
Zn2+ | activation | Oryctolagus cuniculus | |
Zn2+ | can substitute for Mn2+ with 8% | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
separable by gel filtration | Oryctolagus cuniculus |
additional information | - |
two MW-species | Oryctolagus cuniculus |
45000 | - |
x * 45000 + x * 50000 + x * 54000, low molecular weight form, SDS-PAGE, major band: MW 45000 | Oryctolagus cuniculus |
50000 | - |
x * 45000 + x * 50000 + x * 54000, low molecular weight form, SDS-PAGE, major band: MW 45000 | Oryctolagus cuniculus |
54000 | - |
x * 45000 + x * 50000 + x * 54000, low molecular weight form, SDS-PAGE, major band: MW 45000 | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide | Oryctolagus cuniculus | key enzyme of biosynthesis of complex and hybrid N-glycans | UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
two molecular weight forms separable by gel filtration, purification of low-molecular weight form | Oryctolagus cuniculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein] | mechanism | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.8 | - |
low-molecular weight form | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | acts on synthetic substrate analogues | Oryctolagus cuniculus | ? | - |
? | |
additional information | no acceptor substrates are N-acetyl-D-glucosamine, N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-1,4-N-acetyl-D-glucosaminyl-Asn | Oryctolagus cuniculus | ? | - |
? | |
UDP + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosamine | - |
Oryctolagus cuniculus | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | R: H | Oryctolagus cuniculus | UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | R: H | ? | |
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | 1,4-N-acetyl-D-glucosaminyl-Asn | Oryctolagus cuniculus | UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | R: H | ? | |
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | synthetic-beta-D-mannosyl-(1,6-anhydro)-derivative acts as substrate, too | Oryctolagus cuniculus | UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | R: H | ? | |
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide | key enzyme of biosynthesis of complex and hybrid N-glycans | Oryctolagus cuniculus | UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide | - |
? | |
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | substrate specificity | Oryctolagus cuniculus | UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | R: 1,4-N-acetyl-D-glucosaminyl-Asn | ? | |
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | essential for activity: unsubstituted equatorial hydroxyl on C-4 of beta-linked mannosyl-residue | Oryctolagus cuniculus | UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | R: 1,4-N-acetyl-D-glucosaminyl-Asn | ? | |
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | R: 1,4-N-acetyl-D-glucosaminyl-Asn | Oryctolagus cuniculus | UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | R: 1,4-N-acetyl-D-glucosaminyl-Asn | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000 + x * 50000 + x * 54000, low molecular weight form, SDS-PAGE, major band: MW 45000 | Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
- |
Oryctolagus cuniculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.1 | 6.9 | about half-maximal activity at pH 5.1 and about 70% of maximal activity at pH 6.9 | Oryctolagus cuniculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
UDP-N-acetylglucosamine | - |
Oryctolagus cuniculus | |
1.8 | - |
alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
Oryctolagus cuniculus |