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Literature summary for 2.4.1.101 extracted from
- Studies on UDP-N-acetylglucosamine: alpha-mannoside beta-N-acetylglucosaminyltransferase of rat liver and hepatomas (1981), Biochim. Biophys. Acta, 661, 148-157.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | - |
Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.33 | - |
ovalbumin glycopeptide V | transferase A | Rattus norvegicus | |
| 0.44 | - |
ovalbumin | transferase A | Rattus norvegicus | |
| 4.5 | - |
ovalbumin | transferase B | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | transferase B, hepatoma | Rattus norvegicus | 5829 | - |
| membrane | transferase A | Rattus norvegicus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | requirement | Rattus norvegicus | |
| Mn2+ | 2 mM | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male Donryu | - |
| Rattus norvegicus | - |
transferases A and B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Rattus norvegicus |
| two forms: transferase A and B, the latter only in hepatoma | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatoma cell | diethylnitrosamine- or dimethylaminoazobenzene-induced hepatoma, Morris 5123D hepatoma or AH-109A, solid or ascitic | Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000015 | - |
transferase A, liver | Rattus norvegicus |
| 0.00065 | - |
transferase B, hepatoma | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrates: dolichol phosphate, asialo-bovine submaxillary mucin or galactosylated asialo-bovine submaxillary mucin, different specificities of transferase A and B, overview | Rattus norvegicus | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | R: 1,4-N-acetyl-D-glucosaminyl-Asn | Rattus norvegicus | UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | R: 1,4-N-acetyl-D-glucosaminyl-Asn | ? | |
| UDP-N-acetyl-D-glucosamine + asialofetuin | beta-galactosidase-, beta-N-acetylhexosamidase- and alpha-mannosidase-treated asialofetuin, beta-galactosidase- and beta-N-acetylhexosamidase-treated asialofetuin | Rattus norvegicus | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-galactosidase- and beta-N-acetylhexosamidase-treated asialotransferrin | - |
Rattus norvegicus | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + ovalbumin | - |
Rattus norvegicus | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + ovalbumin glycopeptide V | best substrate | Rattus norvegicus | UDP + ovalbumin glycopeptide IIIA | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.3 | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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