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Literature summary for 2.4.1.100 extracted from
- Purification and characterization of a fructosyltransferase from onion bulbs and its key role in the synthesis of fructo-oligosaccharides in vivo (2005), New Phytol., 165, 513-524.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sodium deoxycholate | - |
Allium cepa |  |
| Triton X-100 | - |
Allium cepa | |
| Tween-80 | - |
Allium cepa | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, 100% inhibition | Allium cepa | |
| CuSO4 | 1 mM, 22% inhibition | Allium cepa | |
| EDTA | 1 mM, 25% inhibition | Allium cepa | |
| Hg2+ | 1 mM, 100% inhibition | Allium cepa | |
| p-chloromercuribenzoate | 0.1 mM, 97% inhibition | Allium cepa | |
| Sodium dodecyl sulfate | 1 mM, 45% inhibition | Allium cepa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18 | - |
1-kestose | pH 5.5, 30°C | Allium cepa | |
| 440 | - |
nystose | pH 5.5, 30°C | Allium cepa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
gel filtration | Allium cepa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Allium cepa | plays an important role in the synthesis of inulin and inulinneo-series fructo-oligosaccharides in onion bulb | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Allium cepa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Allium cepa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bulb | - |
Allium cepa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.63 | - |
pH 5.5, 30°C | Allium cepa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-kestose | - |
Allium cepa | 1F,6G-di-beta-D-fructofuranosylsucrose + nystose | plus a small amount of neokestose | ? | |
| additional information | plays an important role in the synthesis of inulin and inulinneo-series fructo-oligosaccharides in onion bulb | Allium cepa | ? | - |
? | |
| nystose | - |
Allium cepa | 1F(1-beta-D-fructofuranosyl)2-6G-beta-D-fructofuranosysucrose + 1F(1-beta-D-fructofuranosyl)3sucrose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | N-terminal amino acid sequence | Allium cepa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | stable for 15 min | Allium cepa |
| 50 | - |
inactivation above | Allium cepa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | 8 | isoelectric focusing | Allium cepa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.3 | 6.3 | 30°C, stable for 30 min | Allium cepa |
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