Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.10 extracted from

  • Desai, M.; Patel, K.
    Isolation, optimization, and purification of extracellular levansucrase from nonpathogenic Klebsiella strain L1 isolated from waste sugarcane bagasse (2019), Biocatal. Agricult. Biotechnol., 19, 101107 .
No PubMed abstract available
Search for more literature for 2.4.1.10

Activating Compound

Activating Compound Comment Organism Structure
NaCl maximum activity at 2% (w/v) Klebsiella sp. L1
sucrose sucrose concentration at 10% (w/v) is the excellent levansucrase inducer Klebsiella sp. L1

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Klebsiella sp. L1
-
-

Organism

Organism UniProt Comment Textmining
Klebsiella sp. L1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-cellulose column chromatography and Sephadex G-100 gel filtration Klebsiella sp. L1

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.83
-
 crude enzyme, at pH 5.0 and 40°C Klebsiella sp. L1
48.19
-
 after 5.45fold purification, at pH 5.0 and 40°C Klebsiella sp. L1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + H2O
-
 Klebsiella sp. L1 glucose + fructose + levan + sucrose + fructooligosaccharides
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Klebsiella sp. L1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Klebsiella sp. L1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Klebsiella sp. L1