Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM EDTA enhances the enzyme activity by 31% | Halomonas smyrnensis | |
NaCl | the enzyme requires at least 1.5 M NaCl to be active and shows maximum activity in the presence of 3.5 M NaCl | Halomonas smyrnensis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Halomonas smyrnensis |
General Stability | Organism |
---|---|
the enzyme is still very active at salt concentrations close to saturation (4.5 M NaCl) | Halomonas smyrnensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | complete inhibition at 5 mM | Halomonas smyrnensis | |
Ca2+ | 1% residual activity at 1 mM | Halomonas smyrnensis | |
Co2+ | complete inhibition at 1 mM | Halomonas smyrnensis | |
Cu2+ | complete inhibition at 1 mM | Halomonas smyrnensis | |
Fe2+ | complete inhibition at 1 mM | Halomonas smyrnensis | |
KI | - |
Halomonas smyrnensis | |
Mg2+ | 61% residual activity at 5 mM | Halomonas smyrnensis | |
Mn2+ | 6% residual activity at 5 mM | Halomonas smyrnensis | |
Na2S2O5 | - |
Halomonas smyrnensis | |
NaBr | - |
Halomonas smyrnensis | |
NaNO3 | - |
Halomonas smyrnensis | |
Ni2+ | complete inhibition at 1 mM | Halomonas smyrnensis | |
SDS | 2% residual activity at 5 mM | Halomonas smyrnensis | |
Triton X-100 | 71% residual activity at 5 mM | Halomonas smyrnensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halomonas smyrnensis | M1QZ44 | - |
- |
Halomonas smyrnensis AAD6 | M1QZ44 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Halomonas smyrnensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme cannot hydrolyze levan from Halomonas smyrnensis, Zymomonas mobilis and Bacillus subtilis, and inulin from chicory | Halomonas smyrnensis | ? | - |
- |
|
additional information | the enzyme cannot hydrolyze levan from Halomonas smyrnensis, Zymomonas mobilis and Bacillus subtilis, and inulin from chicory | Halomonas smyrnensis AAD6 | ? | - |
- |
|
sucrose | the reaction occurs only in the presence of high salt concentrations (more than 1.5 M NaCl) | Halomonas smyrnensis | levan + fructooligosaccharides | - |
? | |
sucrose | the reaction occurs only in the presence of high salt concentrations (more than 1.5 M NaCl) | Halomonas smyrnensis AAD6 | levan + fructooligosaccharides | - |
? | |
sucrose + cellobiose | - |
Halomonas smyrnensis | fructosylated cellobiose + ? | - |
? | |
sucrose + cellobiose | - |
Halomonas smyrnensis AAD6 | fructosylated cellobiose + ? | - |
? | |
sucrose + galactose | - |
Halomonas smyrnensis | Fru(beta-2,6)Gal + ? | - |
? | |
sucrose + galactose | - |
Halomonas smyrnensis AAD6 | Fru(beta-2,6)Gal + ? | - |
? | |
sucrose + glucose | - |
Halomonas smyrnensis | 1-kestotriose + 6-kestotriose + 1-kestotetraose + ? | - |
? | |
sucrose + glucose | - |
Halomonas smyrnensis AAD6 | 1-kestotriose + 6-kestotriose + 1-kestotetraose + ? | - |
? | |
sucrose + L-arabinose | - |
Halomonas smyrnensis | 6-kestotriose + ? | - |
? | |
sucrose + L-arabinose | - |
Halomonas smyrnensis | 6-kestotriose + fructosylated L-arabinose | - |
? | |
sucrose + lactose | - |
Halomonas smyrnensis | fructosylated lactose + ? | - |
? | |
sucrose + raffinose | - |
Halomonas smyrnensis | Fru(beta-2,1)Gal + fructosylated lactose + fructosylated cellobiose + 1-kestotriose + 6-kestotriose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Halomonas smyrnensis |
? | x * 47300, calculated from amino acid sequence | Halomonas smyrnensis |
Synonyms | Comment | Organism |
---|---|---|
Lsc | - |
Halomonas smyrnensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Halomonas smyrnensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.9 | - |
- |
Halomonas smyrnensis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Halomonas smyrnensis | calculated from amino acid sequence | - |
4.6 |