Literature summary for 2.4.1.10 extracted from

Wuerges, J.; Caputi, L.; Cianci, M.; Boivin, S.; Meijers, R.; Benini, S.
The crystal structure of Erwinia amylovora levansucrase provides a snapshot of the products of sucrose hydrolysis trapped into the active site (2015), J. Struct. Biol., 191, 290-298.

Cloned(Commentary)

Cloned (Comment)	Organism
recobinant expression of N-terminally His6- and GST-tagged enzyme in Escherichia coli	Erwinia amylovora

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion method, mixing of 0.001 ml of 25 mg/ml protein solution containing 25 mM Tris-HCl, pH 7.5, 150 mM NaCl, with 0.001 ml of crystallisation reagent containing 35% PEG 2000 MME, 0.1 M KSCN, X-ray diffraction structure determination and analysis at 2.77 A resolution, molecular replacement using Gluconacetobacter diazotrophicus levansucrase LsdA, PDB ID 1W18, as a search model, enzyme structure comparisons, overview	Erwinia amylovora

Crystallization (Comment)

Organism

hanging drop vapour diffusion method, mixing of 0.001 ml of 25 mg/ml protein solution containing 25 mM Tris-HCl, pH 7.5, 150 mM NaCl, with 0.001 ml of crystallisation reagent containing 35% PEG 2000 MME, 0.1 M KSCN, X-ray diffraction structure determination and analysis at 2.77 A resolution, molecular replacement using Gluconacetobacter diazotrophicus levansucrase LsdA, PDB ID 1W18, as a search model, enzyme structure comparisons, overview

Erwinia amylovora

Organism

Organism	UniProt	Comment	Textmining
Erwinia amylovora	Q46654	-	-
Erwinia amylovora ATCC 49946	Q46654	-	-

Subunits

Subunits	Comment	Organism
More	enzyme structure comparisons, overview. The enzyme shows the five-bladed beta-propeller typical of glycoside hydrolase families 32 and 68 members	Erwinia amylovora

Synonyms

Synonyms	Comment	Organism
Lsc	-	Erwinia amylovora
SacB	-	Erwinia amylovora

General Information

General Information	Comment	Organism
evolution	the enzyme shows the five-bladed beta-propeller typical of glycoside hydrolase families 32 and 68 members	Erwinia amylovora
additional information	enzyme structure comparisons, overview	Erwinia amylovora
physiological function	levan is produced by a single enzyme, levansucrase. Levan is required for the formation of a protective biofilm and represents one of several virulence factors of the bacterium	Erwinia amylovora