Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, SDS-PAGE | Zymomonas mobilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zymomonas mobilis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Zymomonas mobilis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | three activities are displayed by the levansucrase from Zymomonas mobilis: formation of levan (polymerization), production of short-chain fructooligosaccharides (FOS), and sucrose hydrolysis. Several inulin-type FOS (1-kestose, nystose, 1F-fructosylnystose), neo-FOS (blastose, neokestose, neonystose) and levan-type FOS (6-kestose, 6,6-nystose) are synthesized by levansucrase, identification by mass spectroscopy and NMR spectrometry, e.g. of 6,6-nystose (i.e. Fru-beta(2->6)-Fru-beta(2->6)-Fru-beta(2->1)-alphaGlc). Assay method optimization, transfructosylation to hydrolysis ratio. , overview | Zymomonas mobilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Zymomonas mobilis |
Synonyms | Comment | Organism |
---|---|---|
LEV-Y | - |
Zymomonas mobilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 40 | assay at, levan formation reaches the highest value at 4°C and 100 g/l sucrose. The increase of temperature to 40°C and fructose concentration of 600 g/l causes a significant decrease of the levan concentration and a higher production of short-chain fructooligosaccharides, an increase of the temperature also causes an enhancement of hydrolytic activity | Zymomonas mobilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | activity range, profile overview | Zymomonas mobilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Zymomonas mobilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | activity range, no activity below pH 3.5 and above pH 6.5, profile, overview | Zymomonas mobilis |