Literature summary for 2.4.1.10 extracted from
Szwengiel, A.; Czarnecka, M.; Czarnecki, Z.
Levan synthesis during associated action of levansucrase and Candida cacaoi DSM 2226 yeast (2007), Pol. J. Food Nutr. Sci., 57, 433-440.
No PubMed abstract available
Application
Application |
Comment |
Organism |
synthesis |
D-glucose acts as an inhibitor of the transfructosylation reaction, Candida cacaoi selectively removes glucose from the reaction medium, the decrease of glucose concentration by yeast in the medium by 16-19% results in the increased degree of levan polymerisation (by 6 to 9%) and efficiency of levan synthesis (by 9 to 11%) |
Bacillus subtilis |
General Stability
General Stability |
Organism |
levansucrase shows high stability during 140 h synthesis at 37°C |
Bacillus subtilis |
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
D-glucose |
D-glucose acts as an inhibitor of the transfructosylation reaction, the decrease of glucose concentration by yeast in the medium by 16-19% results in the increased degree of levan polymerisation (by 6 to 9%) and efficiency of levan synthesis (by 9 to 11%) |
Bacillus subtilis |
|
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
Mn2+ |
The transferase activity of levansucrase in the reaction mixture supplemented with Mn2+ is 100% higher than the enzyme activity in medium without metal ions, the hydrolytic activity of the levansucrase is lowered by 80% |
Bacillus subtilis |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Bacillus subtilis |
- |
DSM 347 |
- |
Source Tissue
Source Tissue |
Comment |
Organism |
Textmining |
culture supernatant |
ethanol precipitation |
Bacillus subtilis |
- |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
sucrose + (2,6-beta-D-fructosyl)n |
reduction of glucose by yeasts (Candida cacaoi DSM 2226), introduced into a dialysing membrane, situated in the reaction medium, and the presence of Mn2+ results in an increase of levan synthesis efficiency to 64% |
Bacillus subtilis |
D-glucose + (2,6-beta-D-fructosyl)n+1 |
- |
? |
|