Cloned (Comment) | Organism |
---|---|
cloning and expression of LsdA in a levansucrase deficient Acetobacter diazotrophicus mutant strain | Gluconacetobacter diazotrophicus |
Protein Variants | Comment | Organism |
---|---|---|
D309N | site-directed mutagenesis of RDP-motif, no affection of enzyme secretion but hydrolysis activity, 13fold increase of kcat, 71fold decrease of kcat/Km, unaltered Km for sucrose | Gluconacetobacter diazotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.5 | - |
sucrose | mutant D309N | Gluconacetobacter diazotrophicus | |
11.4 | - |
sucrose | wild-type enzyme | Gluconacetobacter diazotrophicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Gluconacetobacter diazotrophicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
alignment of RDP-motif sequence | Gluconacetobacter diazotrophicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconacetobacter diazotrophicus | Q43998 | i.e. Acetobacter diazotrophicus | - |
Gluconacetobacter diazotrophicus | Q43998 | constitutive LsdA, strain SRT4 | - |
Purification (Comment) | Organism |
---|---|
recombinant and native wild-type and recombinant mutant from supernatant | Gluconacetobacter diazotrophicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside | Asp309 is involved in catalytic ping-pong mechanism, localization in conserved RDP-motif | Gluconacetobacter diazotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,6-beta-D-fructosyl)n | beta-D-fructofuranosidase activity | Gluconacetobacter diazotrophicus | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
sucrose | wild-type enzyme | Gluconacetobacter diazotrophicus | |
13.3 | - |
sucrose | mutant D309N | Gluconacetobacter diazotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
wild-type and mutant D309N | Gluconacetobacter diazotrophicus |