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Literature summary for 2.4.1.10 extracted from

  • Hernandez, L.; Arrieta, J.; Betancourt, L.; Falcon, V.; Madrazo, J.; Coego, A.; Menendez, C.
    Levansucrase from Acetobacter diazotrophicus SRT4 is secreted via periplasm by a signal-peptide-dependent pathway (1999), Curr. Microbiol., 39, 146-152.
    View publication on PubMed
Search for more literature for 2.4.1.10

Activating Compound

Activating Compound Comment Organism Structure
Sodium dodecyl sulfate slight activation at 5% w/v Gluconacetobacter diazotrophicus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Acetobacter azotrophicus wild-type strain SRT4, 6fold increase in periplasm, 1.7fold increase of extracellular enzyme Gluconacetobacter diazotrophicus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol slight inhibition at 50 mM Gluconacetobacter diazotrophicus
dithiothreitol weak inhibition at 50 mM Gluconacetobacter diazotrophicus
proteinase K 90% remaining activity after incubation at 37°C for 1 h Gluconacetobacter diazotrophicus
Urea weak inhibition at 5 M Gluconacetobacter diazotrophicus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular signal peptide dependent secretion mechanism Gluconacetobacter diazotrophicus
-
-
periplasm precursor form, before secretion into the medium, maturation includes cleavage of 30 amino acid residue signal peptide and formation of a pyroglutamic acid residue, not attached to membranes Gluconacetobacter diazotrophicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, SDS-PAGE Gluconacetobacter diazotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + (2,6-beta-D-fructosyl)n Gluconacetobacter diazotrophicus translocation of the enzyme is a rate-limiting step glucose + (2,6-beta-D-fructosyl)n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Gluconacetobacter diazotrophicus
-
 i.e. Acetobacter diazotrophicus
-
Gluconacetobacter diazotrophicus
-
 constitutive LsdA, strain SRT4
-

Purification (Commentary)

Purification (Comment) Organism
extracellular and periplasmic forms Gluconacetobacter diazotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2561
-
 purified periplasmic enzyme Gluconacetobacter diazotrophicus
2603
-
 purified extracellular enzyme Gluconacetobacter diazotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (2,6-beta-D-fructosyl)n
-
 Gluconacetobacter diazotrophicus glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
sucrose + (2,6-beta-D-fructosyl)n translocation of the enzyme is a rate-limiting step Gluconacetobacter diazotrophicus glucose + (2,6-beta-D-fructosyl)n+1
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Gluconacetobacter diazotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Gluconacetobacter diazotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
 assay at Gluconacetobacter diazotrophicus