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Literature summary for 2.4.1.10 extracted from

  • Hettwer, U.; Gross, M.; Rudolph, K.
    Purification and characterization of an extracellular levansucrase from Pseudomonas syringae pv. phaseolicola (1995), J. Bacteriol., 177, 2834-2839.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
elugent slight activation at 1% Pseudomonas savastanoi pv. phaseolicola
Tween 20 slight activation at 1% Pseudomonas savastanoi pv. phaseolicola

General Stability

General Stability Organism
1% deoxycholate reduces the activity by 4%, 10% SDS decreases the activity by 27% Pseudomonas savastanoi pv. phaseolicola

Inhibitors

Inhibitors Comment Organism Structure
D-glucose
-
Bacillus subtilis
D-glucose complete inhibition at 30 mM Pseudomonas savastanoi pv. phaseolicola
D-glucose complete inhibition at 30 mM Zymomonas mobilis
mannose no inhibition Pseudomonas savastanoi pv. phaseolicola
additional information arabinose is not inhibitory Pseudomonas savastanoi pv. phaseolicola
additional information
-
Zymomonas mobilis
NaCl 30% of the protein is precipitated at 2 M NaCl Pseudomonas savastanoi pv. phaseolicola
proteinase K proteinase K has no effect Pseudomonas savastanoi pv. phaseolicola
Urea 6 M urea decreased the activity by 40% Pseudomonas savastanoi pv. phaseolicola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
160
-
sucrose
-
Pseudomonas savastanoi pv. phaseolicola

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus subtilis
-
-
extracellular
-
Zymomonas mobilis
-
-
extracellular
-
Pseudomonas savastanoi pv. phaseolicola
-
-
extracellular
-
Streptococcus mutans
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
amino acid N-terminal sequence alignment Bacillus subtilis
additional information
-
amino acid N-terminal sequence alignment Zymomonas mobilis
additional information
-
amino acid N-terminal sequence alignment Pseudomonas savastanoi pv. phaseolicola
additional information
-
amino acid N-terminal sequence alignment Streptococcus mutans
additional information
-
amino acid N-terminal sequence alignment Erwinia amylovora
45000
-
x * 45000, SDS-PAGE Pseudomonas savastanoi pv. phaseolicola
68000
-
gel filtration Pseudomonas savastanoi pv. phaseolicola

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Erwinia amylovora
-
-
-
Pseudomonas savastanoi pv. phaseolicola
-
-
-
Streptococcus mutans
-
-
-
Zymomonas mobilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas savastanoi pv. phaseolicola

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
298
-
purified enzyme Pseudomonas savastanoi pv. phaseolicola

Storage Stability

Storage Stability Organism
4°C, no loss of activity, 6 months Pseudomonas savastanoi pv. phaseolicola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (2,6-beta-D-fructosyl)n
-
Bacillus subtilis glucose + (2,6-beta-D-fructosyl)n+1
-
r
sucrose + (2,6-beta-D-fructosyl)n
-
Zymomonas mobilis glucose + (2,6-beta-D-fructosyl)n+1
-
r
sucrose + (2,6-beta-D-fructosyl)n
-
Streptococcus mutans glucose + (2,6-beta-D-fructosyl)n+1
-
?
sucrose + (2,6-beta-D-fructosyl)n
-
Pseudomonas savastanoi pv. phaseolicola glucose + (2,6-beta-D-fructosyl)n+1 molecular weight of levan product is dependent on temperature, salinity, and sucrose concentration r

Subunits

Subunits Comment Organism
?
-
Bacillus subtilis
? x * 45000, SDS-PAGE Pseudomonas savastanoi pv. phaseolicola

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
18
-
levan synthesis Pseudomonas savastanoi pv. phaseolicola
60
-
sucrose hydrolysis Pseudomonas savastanoi pv. phaseolicola

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
18 60
-
Pseudomonas savastanoi pv. phaseolicola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Bacillus subtilis
6
-
-
Pseudomonas savastanoi pv. phaseolicola

pH Range

pH Minimum pH Maximum Comment Organism
4.8 6.8 about half-maximal activity at pH 4.8 and pH 6.8 Pseudomonas savastanoi pv. phaseolicola