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Literature summary for 2.4.1.10 extracted from

  • Yanase, H.; Iwata, M.; Nakahigashi, R.; Kita, K.; Kato, N.; Tonomura, K.
    Purification, crystallization, and properties of the extracellular levansucrase from Zymomonas mobilis (1992), Biosci. Biotechnol. Biochem., 56, 1335-1337.
No PubMed abstract available
Search for more literature for 2.4.1.10

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization from saturated (NH4)2SO4-solution, pH 6.0, 4°C, 20 mM potassium phosphate, 0.2 M NaCl Zymomonas mobilis

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Zymomonas mobilis
Cu2+
-
 Zymomonas mobilis
Hg2+
-
 Zymomonas mobilis
Mersalyl acid
-
 Zymomonas mobilis
additional information not: EDTA Zymomonas mobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
122
-
 sucrose
-
 Zymomonas mobilis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Zymomonas mobilis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 2 * 56000, SDS-PAGE Zymomonas mobilis
94000
-
 gel filtration Zymomonas mobilis

Organism

Organism UniProt Comment Textmining
Zymomonas mobilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Zymomonas mobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (2,6-beta-D-fructosyl)n at lower temperatures, such as 5°C and 15°C and pH 4.0-6.0 the transfructosylation is preferentially catalyzed rather than the hydrolysis of sucrose, but inversely at higher temperatures such as 30°C and 40°C and pH 7.0-8.0 the hydrolysis is preferentially catalyzed Zymomonas mobilis glucose + (2,6-beta-D-fructosyl)n+1
-
 r
sucrose + (2,6-beta-D-fructosyl)n enzyme catalyzes the liberation of reducing sugars from substrates having 2-beta-D-fructofuranose residues on a terminal such as sucrose, raffinose, levan from Aerobacter levanicum and inulin with a relative activity of 100:104:1:0.01 Zymomonas mobilis glucose + (2,6-beta-D-fructosyl)n+1
-
 r
sucrose + H2O hydrolysis Zymomonas mobilis D-glucose + D-fructose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 56000, SDS-PAGE Zymomonas mobilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 at lower temperatures, such as 4°C and 15°C, the transfructosylation is preferentially catalyzed rather than the hydrolysis of sucrose, but inversely at higher temperatures such as 30°C and 40°C the hydrolysis is preferentially catalyzed Zymomonas mobilis
30
-
 assay at Zymomonas mobilis
50
-
-
 Zymomonas mobilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 pH 6.0, 30 min Zymomonas mobilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 6
-
 Zymomonas mobilis

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 15°C, 10% sucrose, pH 4.0-6.0: transfructosylation preferentially occurs and oligo- and polysaccharides are produced, pH 7.0-8.0: hydrolysis of sucrose is enhanced, oligosaccharides are produced rather than polysaccharides as transfructosylation products Zymomonas mobilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 6 30°C, 24 h, stable Zymomonas mobilis