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Literature summary for 2.4.1.1 extracted from

  • Zhao, N.; Hou, M.; Wang, T.; Chen, Y.; Lv, Y.; Li, Z.; Zhang, R.; Xin, W.; Zou, X.; Hou, L.
    Cloning and expression patterns of the brine shrimp (Artemia sinica) glycogen phosphorylase (GPase) gene during development and in response to temperature stress (2014), Mol. Biol. Rep., 41, 9-18.
    View publication on PubMed
Search for more literature for 2.4.1.1

Cloned(Commentary)

Cloned (Comment) Organism
GPase gene, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, expression in Escherichia coli strain DH5alpha Artemia sinica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information an allosteric enzyme existing as two interconvertible forms: phosphorylase a (active type) and phosphorylase b (inactive type) Artemia sinica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Artemia sinica 5737
-
membrane
-
 Artemia sinica 16020
-
additional information prediction of protein subcellular localization of the GPase indicates that 52.3% is located in the cytoplasm, 21.7% in the nucleus, 17.4% in the mitochondria and 4.3% in the cytoskeleton and plasma membrane Artemia sinica
-
-
nucleus
-
 Artemia sinica 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92500
-
 2 * 92500, SDS-PAGE, 2 * 98190, sequence calculation Artemia sinica
98190
-
 2 * 92500, SDS-PAGE, 2 * 98190, sequence calculation Artemia sinica
190000
-
 about Artemia sinica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[(1->4)-alpha-D-glucosyl]n + phosphate Artemia sinica
-
 [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Artemia sinica I3QKR5 collected from a salt lake, YunCheng, Shanxi Province, China
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Artemia sinica
-
cyst
-
 Artemia sinica
-
additional information wide distribution of the GPase gene during different developmental stages, expression analysis, overview Artemia sinica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[(1->4)-alpha-D-glucosyl]n + phosphate
-
 Artemia sinica [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Subunits

Subunits Comment Organism
homodimer 2 * 92500, SDS-PAGE, 2 * 98190, sequence calculation Artemia sinica

Synonyms

Synonyms Comment Organism
alpha-1,4 glucan phosphorylase UniProt Artemia sinica
GPase
-
 Artemia sinica

pI Value

Organism Comment pI Value Maximum pI Value
Artemia sinica sequence calcualtion
-
 5.67

Expression

Organism Comment Expression
Artemia sinica expression patterns of the glycogen phosphorylase gene during development and in response to temperature stress, overview. Expression is downregulated at temperatures ranging from 20°C to 25°C and is subsequently upregulated in the range 5-15°C additional information

General Information

General Information Comment Organism
metabolism glycogen phosphorylase is a key enzyme involved in intracellular glycogen catabolism, catalyzing the first step in glycogen degradation. In the diapause, GPase catalyzes glycogen into the closely related molecule, sorbitol. The GPase gene expression is stress-related and might play an important role in Artemia development and metabolism Artemia sinica