Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.1 extracted from

  • Hong, Y.; Wu, L.; Liu, B.; Peng, C.; Sheng, D.; Ni, J.; Shen, Y.
    Characterization of a glucan phosphorylase from the thermophilic archaeon Sulfolobus tokodaii strain 7 (2008), J. Mol. Catal. B, 54, 27-34.
No PubMed abstract available
Search for more literature for 2.4.1.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Sulfurisphaera tokodaii str. 7

Protein Variants

Protein Variants Comment Organism
H185A kM-valaue for maltotriose is 2.2fold higher than wild-type value, kcat for maltotriose is 34fold lower than wild-type value Sulfurisphaera tokodaii str. 7
K308A kM-valaue for maltotriose is nearly identical to wild-type value, kcat for maltotriose is 225fold lower than wild-type value Sulfurisphaera tokodaii str. 7
R303A kM-valaue for maltotriose is 1.1fold higher than wild-type value, kcat for maltotriose is 155fold lower than wild-type value Sulfurisphaera tokodaii str. 7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 starch pH 6.2, 37°C, phosphorolytic direction, wild-type enzyme Sulfurisphaera tokodaii str. 7
0.045
-
 starch pH 6.2, 37°C, synthetic direction, wild-type enzyme Sulfurisphaera tokodaii str. 7
0.28
-
 maltotetraose pH 6.2, 37°C, phosphorolytic direction, wild-type enzyme Sulfurisphaera tokodaii str. 7
0.3
-
 maltotriose pH 6.2, 37°C, synthetic direction, mutant enzyme k308A Sulfurisphaera tokodaii str. 7
0.31
-
 maltotriose pH 6.2, 37°C, synthetic direction, wild-type enzyme Sulfurisphaera tokodaii str. 7
0.35
-
 maltotriose pH 6.2, 37°C, synthetic direction, mutant enzyme R303A Sulfurisphaera tokodaii str. 7
0.478
-
 maltotetraose pH 6.2, 37°C, synthetic direction, wild-type enzyme Sulfurisphaera tokodaii str. 7
0.69
-
 maltotriose pH 6.2, 37°C, synthetic direction, mutant enzyme H185A Sulfurisphaera tokodaii str. 7
0.79
-
 maltotriose pH 6.2, 37°C, phosphorolytic direction, wild-type enzyme Sulfurisphaera tokodaii str. 7
1.44
-
 maltose pH 6.2, 37°C, synthetic direction, wild-type enzyme Sulfurisphaera tokodaii str. 7

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii str. 7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Sulfurisphaera tokodaii str. 7

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltose + alpha-D-glucose 1-phosphate
-
 Sulfurisphaera tokodaii str. 7 ?
-
 ?
maltotetraose + glucose 1-phosphate
-
 Sulfurisphaera tokodaii str. 7 maltopentaose + phosphate
-
 r
maltotriose + alpha-D-glucose 1-phosphate
-
 Sulfurisphaera tokodaii str. 7 maltotetraose + phosphate
-
 r
starch + glucose 1-phosphate
-
 Sulfurisphaera tokodaii str. 7 starch + phosphate
-
 r
[(1->4)-alpha-D-glucosyl]n + phosphate maltose is the minimum primer for glucan synthesis and maltotriose is the minimum substrate for degradation Sulfurisphaera tokodaii str. 7 [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Synonyms

Synonyms Comment Organism
glucan phosphorylase
-
 Sulfurisphaera tokodaii str. 7
stGP
-
 Sulfurisphaera tokodaii str. 7

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
 at pH 6.0 Sulfurisphaera tokodaii str. 7

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 90 70°C: about 70% of maximal activity, 90°C: 80% of maximal activity Sulfurisphaera tokodaii str. 7

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75 85 stGP retains more than 80% of the full activity after incubation at 75°C and 85°C for 8 h Sulfurisphaera tokodaii str. 7
95
-
 6 h, more than 70% of the full activity remains Sulfurisphaera tokodaii str. 7

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
 maltotriose pH 6.2, 37°C, phosphorolytic direction, wild-type enzyme Sulfurisphaera tokodaii str. 7
0.11
-
 maltotriose pH 6.2, 37°C, synthetic direction, mutant enzyme k308A Sulfurisphaera tokodaii str. 7
0.16
-
 maltotetraose pH 6.2, 37°C, phosphorolytic direction, wild-type enzyme Sulfurisphaera tokodaii str. 7
0.16
-
 maltotriose pH 6.2, 37°C, synthetic direction, mutant enzyme R303A Sulfurisphaera tokodaii str. 7
0.2
-
 starch pH 6.2, 37°C, phosphorolytic direction, wild-type enzyme Sulfurisphaera tokodaii str. 7
0.73
-
 maltotriose pH 6.2, 37°C, synthetic direction, mutant enzyme H185A Sulfurisphaera tokodaii str. 7
15.2
-
 maltose pH 6.2, 37°C, synthetic direction, wild-type enzyme Sulfurisphaera tokodaii str. 7
19.4
-
 starch pH 6.2, 37°C, synthetic direction, wild-type enzyme Sulfurisphaera tokodaii str. 7
24.8
-
 maltotriose pH 6.2, 37°C, synthetic direction, wild-type enzyme Sulfurisphaera tokodaii str. 7
34.6
-
 maltotetraose pH 6.2, 37°C, synthetic direction, wild-type enzyme Sulfurisphaera tokodaii str. 7

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Sulfurisphaera tokodaii str. 7

pH Range

pH Minimum pH Maximum Comment Organism
4 8 pH 4.0: about 60% of maximal activity, pH 8.0: about 50% of maximal activity Sulfurisphaera tokodaii str. 7