Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADPglucose | 2.5 mM, 80% inhibition of stromal phosphorylase | Zea mays | |
diethyldicarbonate | 0.84 mM, 50% inhibition | Zea mays | |
Hg2+ | 1 mM, 77% inhibition of stromal phosphorylase | Zea mays | |
p-chloromercuriphenylsulfonic acid | 1 mM, 88% inhibition of stromal phosphorylase | Zea mays | |
Phenylglyoxal | 12 mM, 50% inhibition | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
amylopectin | stromal phosphorylase, synthesis | Zea mays | |
0.028 | - |
amylopectin | stromal phosphorylase, phosphorolysis | Zea mays | |
0.08 | - |
maltoheptaose | stromal phosphorylase, synthesis | Zea mays | |
0.1 | - |
maltoheptaose | stromal phosphorylase, phosphorolysis | Zea mays | |
0.16 | - |
maltohexaose | stromal phosphorylase, synthesis | Zea mays | |
0.21 | - |
maltohexaose | stromal phosphorylase, phosphorolysis | Zea mays | |
0.23 | - |
maltopentaose | stromal phosphorylase, phosphorolysis | Zea mays | |
0.25 | - |
glycogen | stromal phosphorylase, synthesis | Zea mays | |
0.27 | - |
maltopentaose | stromal phosphorylase, synthesis | Zea mays | |
0.57 | - |
maltotetraose | stromal phosphorylase, synthesis | Zea mays | |
0.94 | - |
glycogen | stromal phosphorylase, phosphorolysis | Zea mays | |
1.51 | - |
glucose 1-phosphate | stromal phosphorylase | Zea mays | |
2.26 | - |
phosphate | stromal phosphorylase | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
amyloplast | stroma | Zea mays | 9501 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
112000 | - |
2 * 112000, stromal phosphorylase, SDS-PAGE | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
stromal phosphorylase, ammonium sulfate, Sephacryl 300 Hr, starch adsorption, Q-Sepharose, Mono Q | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kernel | - |
Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
67.5 | - |
stromal phosphorylase | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + glucose 1-phosphate | - |
Zea mays | amylopectin + phosphate | - |
? | |
glycogen + glucose 1-phosphate | - |
Zea mays | glycogen + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 112000, stromal phosphorylase, SDS-PAGE | Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Zea mays |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stromal phosphorylase, 10% losss of activity after 15 min | Zea mays |
60 | - |
stromal phosphorylase, 93% loss of activity after 15 min | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | phosphorolysis | Zea mays |
5.5 | 6.5 | synthesis | Zea mays |