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Literature summary for 2.4.1.1 extracted from

  • Medicus, R.; Mendicino, J.
    Role of enzyme interactions in the regulation of glycolysis and gluconeogenesis. Purification and properties of the phospho- and dephospho-forms of glycogen phosphorylase from swine kidney (1973), Eur. J. Biochem., 40, 63-75.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
5'-AMP activation Sus scrofa
5'-AMP kidney dephosphorylase is not activated by AMP Sus scrofa
5'-AMP required for dephospho-phosphorylase, kinetics Sus scrofa
phosphate activation Sus scrofa
Phosphorylase kinase activation Sus scrofa
protamine stimulation, dephospho-phosphorylase, kinetics Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
D-glucose 1 mM, significant inhibition of kidney dephosphophosphorylase Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
glycogen kidney phosphophosphorylase Sus scrofa
2.3
-
phosphate kidney phosphophosphorylase, in the presence of 0.8 mg glycogen Sus scrofa
2.6
-
phosphate kidney phosphophosphorylase, in the presence of 0.4 mg glycogen Sus scrofa
2.8
-
phosphate kidney phosphophosphorylase, in the presence of 0.2 mg glycogen Sus scrofa
2.9
-
phosphate kidney phosphophosphorylase, in the presence of 0.1 mg glycogen Sus scrofa
20
-
phosphate glycogen, dephospho-phosphorylase Sus scrofa
30
-
phosphate kidney dephosphophosphorylase, in the presence of 1.6 mg glycogen Sus scrofa
70
-
phosphate kidney dephosphophosphorylase, in the presence of 0.8 mg glycogen Sus scrofa
100
-
phosphate dephospho-phosphorylase Sus scrofa
100
-
phosphate kidney dephosphophosphorylase, in the presence of 0.2 mg glycogen Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[(1->4)-alpha-D-glucosyl]n + phosphate Sus scrofa glycogen metabolism [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification activation by phosphorylation Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
alumina C-gamma gel, calcium phosphate gel, protamine sulfate, acetone, ethanol, ammonium sulfate, DEAE-cellulose, hydroxyapatite Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30
-
kidney dephospho-phosphorylase Sus scrofa
70
-
kidney phospho-phosphorylase Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, partially purified, lyophilized, at least 2 months Sus scrofa
-20°C, purified, lyophilized, 30% loss of activity within 3 months Sus scrofa
3°C, 2 weeks, no loss of activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycogen + glucose 1-phosphate
-
Sus scrofa glycogen + phosphate
-
r
[(1->4)-alpha-D-glucosyl]n + phosphate
-
Sus scrofa [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
r
[(1->4)-alpha-D-glucosyl]n + phosphate glycogen metabolism Sus scrofa [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
?

Subunits

Subunits Comment Organism
dimer SDS-PAGE Sus scrofa