Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5'-AMP | activation | Sus scrofa | |
5'-AMP | kidney dephosphorylase is not activated by AMP | Sus scrofa | |
5'-AMP | required for dephospho-phosphorylase, kinetics | Sus scrofa | |
phosphate | activation | Sus scrofa | |
Phosphorylase kinase | activation | Sus scrofa | |
protamine | stimulation, dephospho-phosphorylase, kinetics | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | 1 mM, significant inhibition of kidney dephosphophosphorylase | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
glycogen | kidney phosphophosphorylase | Sus scrofa | |
2.3 | - |
phosphate | kidney phosphophosphorylase, in the presence of 0.8 mg glycogen | Sus scrofa | |
2.6 | - |
phosphate | kidney phosphophosphorylase, in the presence of 0.4 mg glycogen | Sus scrofa | |
2.8 | - |
phosphate | kidney phosphophosphorylase, in the presence of 0.2 mg glycogen | Sus scrofa | |
2.9 | - |
phosphate | kidney phosphophosphorylase, in the presence of 0.1 mg glycogen | Sus scrofa | |
20 | - |
phosphate | glycogen, dephospho-phosphorylase | Sus scrofa | |
30 | - |
phosphate | kidney dephosphophosphorylase, in the presence of 1.6 mg glycogen | Sus scrofa | |
70 | - |
phosphate | kidney dephosphophosphorylase, in the presence of 0.8 mg glycogen | Sus scrofa | |
100 | - |
phosphate | dephospho-phosphorylase | Sus scrofa | |
100 | - |
phosphate | kidney dephosphophosphorylase, in the presence of 0.2 mg glycogen | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[(1->4)-alpha-D-glucosyl]n + phosphate | Sus scrofa | glycogen metabolism | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | activation by phosphorylation | Sus scrofa |
Purification (Comment) | Organism |
---|---|
alumina C-gamma gel, calcium phosphate gel, protamine sulfate, acetone, ethanol, ammonium sulfate, DEAE-cellulose, hydroxyapatite | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30 | - |
kidney dephospho-phosphorylase | Sus scrofa |
70 | - |
kidney phospho-phosphorylase | Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, partially purified, lyophilized, at least 2 months | Sus scrofa |
-20°C, purified, lyophilized, 30% loss of activity within 3 months | Sus scrofa |
3°C, 2 weeks, no loss of activity | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycogen + glucose 1-phosphate | - |
Sus scrofa | glycogen + phosphate | - |
r | |
[(1->4)-alpha-D-glucosyl]n + phosphate | - |
Sus scrofa | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
[(1->4)-alpha-D-glucosyl]n + phosphate | glycogen metabolism | Sus scrofa | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | SDS-PAGE | Sus scrofa |