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Literature summary for 2.4.1.1 extracted from

  • Steup, M.; Schächtele, C.; Latzko, E.
    Purification of a non-chloroplastic alpha-glucan phosphorylase from spinach leaves (1980), Planta, 148, 168-173.
    View publication on PubMed
Search for more literature for 2.4.1.1

General Stability

General Stability Organism
freezing inactivates completely Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Spinacia oleracea 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
89000
-
 2 * 89000, SDS-PAGE Spinacia oleracea
151000
-
 gel filtration Spinacia oleracea
159000
-
 porosity density gradient electrophoresis Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
 spinach, var. Früremona or Kasperik
-

Purification (Commentary)

Purification (Comment) Organism
non-chloroplastic phosphorylase I, ammonium sulfate, dextrin-Sepharose Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.9
-
-
 Spinacia oleracea

Storage Stability

Storage Stability Organism
4°C, 2 weeks, 10% loss of activity Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[(1->4)-alpha-D-glucosyl]n + phosphate
-
 Spinacia oleracea [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 89000, SDS-PAGE Spinacia oleracea

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate requirement Spinacia oleracea
pyridoxal 5'-phosphate 1 mol/mol subunit Spinacia oleracea