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Literature summary for 2.4.1.1 extracted from

  • Rath, V.L.; Hwang, P.K.; Fletterick, R.J.
    Purification and crystallization of glycogen phosphorylase from Saccharomyces cerevisiae (1992), J. Mol. Biol., 225, 1027-1034.
    View publication on PubMed
Search for more literature for 2.4.1.1

Activating Compound

Activating Compound Comment Organism Structure
phosphate activation Saccharomyces cerevisiae
phosphate yeast and mammalian enzymes are activated by covalent phosphorylation, phosphorylase kinase Saccharomyces cerevisiae
phosphate phosphorylation site: a single Thr-residue in the N-terminal region Saccharomyces cerevisiae
Phosphorylase kinase activation Saccharomyces cerevisiae
Phosphorylase kinase yeast and mammalian enzymes are activated by covalent phosphorylation of a single Thr-residue in the N-terminal region Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor diffusion, crystals diffract to 2.4 A resolution Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
glucose 6-phosphate above 5 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 harbouring plasmid Yep24::GPH1
-
Saccharomyces cerevisiae PH5-3
-
 harbouring plasmid Yep24::GPH1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification activation by phosphorylation Saccharomyces cerevisiae
side-chain modification phosphorylation site: a single Thr-residue in the N-terminal region Saccharomyces cerevisiae
side-chain modification yeast and mammalian enzymes are activated by covalent phosphorylation, phosphorylase kinase Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.7
-
 non-phosphorylated enzyme Saccharomyces cerevisiae
85 90 phosphorylated enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycogen + glucose 1-phosphate favoured reaction Saccharomyces cerevisiae glycogen + phosphate
-
 r
glycogen + glucose 1-phosphate favoured reaction Saccharomyces cerevisiae PH5-3 glycogen + phosphate
-
 r
[(1->4)-alpha-D-glucosyl]n + phosphate
-
 Saccharomyces cerevisiae [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
[(1->4)-alpha-D-glucosyl]n + phosphate
-
 Saccharomyces cerevisiae PH5-3 [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r