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Literature summary for 2.4.1.1 extracted from

  • Cuppoletti, J.; Segel, I.H.
    Glycogen phosphorylase from Neurospora crassa: purification of a high-specific-activity, non-phosphorylated form (1979), J. Bacteriol., 139, 411-417.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.1

Activating Compound

Activating Compound Comment Organism Structure
additional information not activated by 5'-AMP Neurospora crassa

General Stability

General Stability Organism
5'-AMP stabilizes dilute enzyme solutions in media of high ionic strength, e.g. 50 mM ammonium sulfate or MgCl2 Neurospora crassa

Inhibitors

Inhibitors Comment Organism Structure
Mg-ATP reversible by AMP Neurospora crassa
Polyethyleneglycol
-
 Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
 gel filtration or PAGE Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[(1->4)-alpha-D-glucosyl]n + phosphate Neurospora crassa glycogen metabolism [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
 wild-type strain CM
-

Purification (Commentary)

Purification (Comment) Organism
polyethylene glycol 6000, ammonium sulfate, gel filtration Neurospora crassa

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Neurospora crassa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
65
-
-
 Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycogen + glucose 1-phosphate glycogen from shell fish Neurospora crassa glycogen + phosphate
-
 r
[(1->4)-alpha-D-glucosyl]n + phosphate
-
 Neurospora crassa [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
[(1->4)-alpha-D-glucosyl]n + phosphate glycogen metabolism Neurospora crassa [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer SDS-PAGE Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate requirement Neurospora crassa