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Literature summary for 2.4.1.1 extracted from
- Purification and properties of phosphorylase from Phymatotrichum omnivorum (1987), Arch. Biochem. Biophys., 254, 579-585.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | - |
Phymatotrichopsis omnivora |  |
| ADP | - |
Phymatotrichopsis omnivora | |
| ATP | - |
Phymatotrichopsis omnivora | |
| Ba2+ | - |
Phymatotrichopsis omnivora | |
| Ca2+ | 1 mM, stimulation at 10 mM | Phymatotrichopsis omnivora | |
| Co2+ | 1 mM, no inhibition at 10 mM | Phymatotrichopsis omnivora | |
| Cu2+ | 10 mM, complete inhibition | Phymatotrichopsis omnivora | |
| cysteine | 1 mM, 86% inhibition | Phymatotrichopsis omnivora | |
| diethyldithiocarbamate | 1 mM, 94% inhibition | Phymatotrichopsis omnivora | |
| dithiothreitol | 1 mM, 56% inhibition | Phymatotrichopsis omnivora | |
| EDTA | 1 mM, 61% inhibition | Phymatotrichopsis omnivora | |
| EGTA | i.e. ethylene glycol bis(beta-aminoethylether)-N,N'-tetraacetic acid, 1 mM, 53% inhibition | Phymatotrichopsis omnivora | |
| Fe2+ | 10 mM, stimulation at 1 mM | Phymatotrichopsis omnivora | |
| glutathione | - |
Phymatotrichopsis omnivora | |
| iodoacetate | weak inhibition | Phymatotrichopsis omnivora | |
| Mn2+ | - |
Phymatotrichopsis omnivora | |
| Zn2+ | strong inhibition | Phymatotrichopsis omnivora | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 0.36 mg/ml, glycogen | Phymatotrichopsis omnivora |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Phymatotrichopsis omnivora | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates at 10 mM, inhibits at 1 mM | Phymatotrichopsis omnivora | |
| Fe2+ | stimulates at 1 mM, inhibits at 10 mM | Phymatotrichopsis omnivora | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 119000 | - |
gel filtration | Phymatotrichopsis omnivora |
| 145000 | - |
x * 145000, SDS-PAGE | Phymatotrichopsis omnivora |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phymatotrichopsis omnivora | - |
- |
- |
| Phymatotrichopsis omnivora Shear Dugg | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, Sephacryl S-200, DEAE-cellulose | Phymatotrichopsis omnivora |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Phymatotrichopsis omnivora | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 222 | - |
- |
Phymatotrichopsis omnivora |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 30 days, 20% loss of activity | Phymatotrichopsis omnivora |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (dextrin)n-1 + glucose 1-phosphate | corn dextrin, phosphorolysis at 85% the rate of glycogen phosphorolysis | Phymatotrichopsis omnivora | (dextrin)n + phosphate | - |
r | |
| (dextrin)n-1 + glucose 1-phosphate | corn dextrin, phosphorolysis at 85% the rate of glycogen phosphorolysis | Phymatotrichopsis omnivora Shear Dugg | (dextrin)n + phosphate | - |
r | |
| glycogen + glucose 1-phosphate | best substrate | Phymatotrichopsis omnivora | glycogen + phosphate | - |
? | |
| glycogen + glucose 1-phosphate | best substrate | Phymatotrichopsis omnivora Shear Dugg | glycogen + phosphate | - |
? | |
| starch + glucose 1-phosphate | phosphorolysis at 31% the rate of glycogen phosphorolysis | Phymatotrichopsis omnivora | starch + phosphate | - |
r | |
| starch + glucose 1-phosphate | phosphorolysis at 31% the rate of glycogen phosphorolysis | Phymatotrichopsis omnivora Shear Dugg | starch + phosphate | - |
r | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | - |
Phymatotrichopsis omnivora | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | - |
Phymatotrichopsis omnivora Shear Dugg | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 145000, SDS-PAGE | Phymatotrichopsis omnivora |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
15 min, slight stimulation | Phymatotrichopsis omnivora |
| 40 | - |
15 min, 16% loss of activity | Phymatotrichopsis omnivora |
| 70 | - |
15 min, complete inactivation | Phymatotrichopsis omnivora |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Phymatotrichopsis omnivora |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | 8 | approx. half-maximal activity at pH 5.6 and approx. 65% of maximal activity at pH 8, citrate/phosphate-buffer | Phymatotrichopsis omnivora |
| 6 | 8.6 | approx. 70% of maximal activity at pH 6 and about half-maximal activity at pH 8.6, Tris-buffer | Phymatotrichopsis omnivora |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | stable | Phymatotrichopsis omnivora |
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