Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | - |
Phymatotrichopsis omnivora | |
ADP | - |
Phymatotrichopsis omnivora | |
ATP | - |
Phymatotrichopsis omnivora | |
Ba2+ | - |
Phymatotrichopsis omnivora | |
Ca2+ | 1 mM, stimulation at 10 mM | Phymatotrichopsis omnivora | |
Co2+ | 1 mM, no inhibition at 10 mM | Phymatotrichopsis omnivora | |
Cu2+ | 10 mM, complete inhibition | Phymatotrichopsis omnivora | |
cysteine | 1 mM, 86% inhibition | Phymatotrichopsis omnivora | |
diethyldithiocarbamate | 1 mM, 94% inhibition | Phymatotrichopsis omnivora | |
dithiothreitol | 1 mM, 56% inhibition | Phymatotrichopsis omnivora | |
EDTA | 1 mM, 61% inhibition | Phymatotrichopsis omnivora | |
EGTA | i.e. ethylene glycol bis(beta-aminoethylether)-N,N'-tetraacetic acid, 1 mM, 53% inhibition | Phymatotrichopsis omnivora | |
Fe2+ | 10 mM, stimulation at 1 mM | Phymatotrichopsis omnivora | |
glutathione | - |
Phymatotrichopsis omnivora | |
iodoacetate | weak inhibition | Phymatotrichopsis omnivora | |
Mn2+ | - |
Phymatotrichopsis omnivora | |
Zn2+ | strong inhibition | Phymatotrichopsis omnivora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 0.36 mg/ml, glycogen | Phymatotrichopsis omnivora |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Phymatotrichopsis omnivora | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates at 10 mM, inhibits at 1 mM | Phymatotrichopsis omnivora | |
Fe2+ | stimulates at 1 mM, inhibits at 10 mM | Phymatotrichopsis omnivora |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
119000 | - |
gel filtration | Phymatotrichopsis omnivora |
145000 | - |
x * 145000, SDS-PAGE | Phymatotrichopsis omnivora |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phymatotrichopsis omnivora | - |
- |
- |
Phymatotrichopsis omnivora Shear Dugg | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Sephacryl S-200, DEAE-cellulose | Phymatotrichopsis omnivora |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Phymatotrichopsis omnivora | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
222 | - |
- |
Phymatotrichopsis omnivora |
Storage Stability | Organism |
---|---|
4°C, 30 days, 20% loss of activity | Phymatotrichopsis omnivora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(dextrin)n-1 + glucose 1-phosphate | corn dextrin, phosphorolysis at 85% the rate of glycogen phosphorolysis | Phymatotrichopsis omnivora | (dextrin)n + phosphate | - |
r | |
(dextrin)n-1 + glucose 1-phosphate | corn dextrin, phosphorolysis at 85% the rate of glycogen phosphorolysis | Phymatotrichopsis omnivora Shear Dugg | (dextrin)n + phosphate | - |
r | |
glycogen + glucose 1-phosphate | best substrate | Phymatotrichopsis omnivora | glycogen + phosphate | - |
? | |
glycogen + glucose 1-phosphate | best substrate | Phymatotrichopsis omnivora Shear Dugg | glycogen + phosphate | - |
? | |
starch + glucose 1-phosphate | phosphorolysis at 31% the rate of glycogen phosphorolysis | Phymatotrichopsis omnivora | starch + phosphate | - |
r | |
starch + glucose 1-phosphate | phosphorolysis at 31% the rate of glycogen phosphorolysis | Phymatotrichopsis omnivora Shear Dugg | starch + phosphate | - |
r | |
[(1->4)-alpha-D-glucosyl]n + phosphate | - |
Phymatotrichopsis omnivora | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
[(1->4)-alpha-D-glucosyl]n + phosphate | - |
Phymatotrichopsis omnivora Shear Dugg | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 145000, SDS-PAGE | Phymatotrichopsis omnivora |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
15 min, slight stimulation | Phymatotrichopsis omnivora |
40 | - |
15 min, 16% loss of activity | Phymatotrichopsis omnivora |
70 | - |
15 min, complete inactivation | Phymatotrichopsis omnivora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Phymatotrichopsis omnivora |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.6 | 8 | approx. half-maximal activity at pH 5.6 and approx. 65% of maximal activity at pH 8, citrate/phosphate-buffer | Phymatotrichopsis omnivora |
6 | 8.6 | approx. 70% of maximal activity at pH 6 and about half-maximal activity at pH 8.6, Tris-buffer | Phymatotrichopsis omnivora |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | stable | Phymatotrichopsis omnivora |