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Literature summary for 2.4.1.1 extracted from

  • Khandelwal, R.L.; Spearman, T.N.; Hamilton, I.R.
    Purification and properties of glycogen phosphorylase from Streptococcus salivarius (1973), Arch. Biochem. Biophys., 154, 295-305.
    View publication on PubMed
Search for more literature for 2.4.1.1

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activation, 5 mM Streptococcus salivarius
cysteine activation Streptococcus salivarius
Mercaptoacetic acid 1 mM, activation, inhibition at 5 mM Streptococcus salivarius
p-hydroxymercuribenzoate slight activation, 0.1-1.0 mM Streptococcus salivarius

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ weak Streptococcus salivarius
Co2+
-
 Streptococcus salivarius
Cu2+ 2 mM, 98% inhibition Streptococcus salivarius
DTNB 1 mM, weak Streptococcus salivarius
Fe2+ 2 mM, 60% inhibition in the presence of 1 mM AMP Streptococcus salivarius
Mg2+ slight Streptococcus salivarius
Mn2+
-
 Streptococcus salivarius
additional information not inhibited by 2-mercaptoethanol; not inhibited by Sr2+ Streptococcus salivarius
Sn2+
-
 Streptococcus salivarius
Zn2+ 2 mM, 63% inhibition in the presence of 1 mM AMP Streptococcus salivarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information 20 mg/ml, oyster glycogen, phosphorolysis, 25 mg/ml, endogen glycogen, synthesis, 27 mg/ml, corn dextrin, phosphorolysis, 40 mg/ml, corn dextrin, synthesis Streptococcus salivarius
additional information
-
 additional information 42 mg/ml, oyster glycogen, synthesis Streptococcus salivarius
3.4
-
 glucose 1-phosphate glycogen synthesis Streptococcus salivarius
3.4
-
 Glucose-1-phosphate glycogen synthesis Streptococcus salivarius
20
-
 phosphate phosphorolysis of oyster glycogen Streptococcus salivarius
25
-
 phosphate phosphorolysis of corn dextrin Streptococcus salivarius

Organism

Organism UniProt Comment Textmining
Streptococcus salivarius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protamine sulfate, ammonium sulfate, DEAE-Sephadex, Sephadex G-200 Streptococcus salivarius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.005
-
-
 Streptococcus salivarius

Storage Stability

Storage Stability Organism
-10°C, 200 mM NaF, 1 month, no loss of activity Streptococcus salivarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(dextrin)n-1 + glucose 1-phosphate best substrate Streptococcus salivarius (dextrin)n + phosphate
-
 r
(dextrin)n-1 + glucose 1-phosphate corn dextrin Streptococcus salivarius (dextrin)n + phosphate
-
 r
glycogen + glucose 1-phosphate about half as effective as dextrin Streptococcus salivarius glycogen + phosphate
-
 r
glycogen + glucose 1-phosphate glycogen from oyster Streptococcus salivarius glycogen + phosphate
-
 r
starch + glucose 1-phosphate glucosylation at 32% the rate of dextrin glucosylation Streptococcus salivarius starch + phosphate
-
 ?
[(1->4)-alpha-D-glucosyl]n + phosphate
-
 Streptococcus salivarius [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 2 h stable Streptococcus salivarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Streptococcus salivarius

pH Range

pH Minimum pH Maximum Comment Organism
5.7 7.2 approx. half-maximal activity at pH 5.7 and 7.2 Streptococcus salivarius