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Literature summary for 2.3.3.9 extracted from
- Kinetic and chemical mechanism of malate synthase from Mycobacterium tuberculosis (2011), Biochemistry, 50, 6879-6887.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli T7 Express cells | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C619S | the mutant exhibits kinetics comparable to those of the wild type enzyme | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dethio-CoA | uncompetitive inhibitor versus glyoxylate and competitive inhibitor versus acetyl-CoA | Mycobacterium tuberculosis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
acetyl-CoA | wild type enzyme, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
| 0.03 | - |
glyoxylate | wild type enzyme, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | cofactor | Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
x * 80000, His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WK17 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WK17 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography and Superdex 200 gel filtration | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + acetyl-CoA + H2O | - |
Mycobacterium tuberculosis | (S)-malate + CoA | - |
? | |
| glyoxylate + acetyl-CoA + H2O | - |
Mycobacterium tuberculosis H37Rv | (S)-malate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 80000, His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| malate synthase G | - |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 23 | - |
acetyl-CoA | wild type enzyme, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
| 23 | - |
glyoxylate | wild type enzyme, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | 8.5 | - |
Mycobacterium tuberculosis |
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