Cloned (Comment) | Organism |
---|---|
wild type enzyme is expressed in Escherichia coli BL21(DE3) cells, mutant enzymes are expressed in Escherichia coli ArcticExpress(DE3) RP cells | Euglena gracilis |
Protein Variants | Comment | Organism |
---|---|---|
D475N | the glyoxylate cycle enzyme mutant completely loses malate synthase activity but retains isocitrate lyase activity | Euglena gracilis |
additional information | the C-terminal domain of the glyoxylate cycle enzyme, when expressed alone (GCE(566-1165)), does not show any enzyme activity | Euglena gracilis |
R187K | the glyoxylate cycle enzyme mutant completely loses malate synthase activity but retains isocitrate lyase activity | Euglena gracilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
glyoxylate | wild type full-length glyoxylate cycle enzyme, at 25°C, pH not specified in the publication | Euglena gracilis | |
0.073 | - |
glyoxylate | N-terminal malate synthase-active domain GCE(13-573) of glyoxylate cycle enzyme, at 25°C, pH not specified in the publication | Euglena gracilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
x * 62000, the 62000 Da N-terminal domain of glyoxylate cycle enzyme provides malate synthase activity, SDS-PAGE | Euglena gracilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE Sepharose column chromatography, HisTrap HP column chromatography, and Superdex 200 pg gel filtration | Euglena gracilis |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + acetyl-CoA + H2O | - |
Euglena gracilis | (S)-malate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62000, the 62000 Da N-terminal domain of glyoxylate cycle enzyme provides malate synthase activity, SDS-PAGE | Euglena gracilis |
Synonyms | Comment | Organism |
---|---|---|
GCE | - |
Euglena gracilis |
GCE(13-573) | N-terminal malate synthase-active domain of glyoxylate cycle enzyme | Euglena gracilis |
glyoxylate cycle enzyme | bifunctional enzyme possessing malate synthase and isocitrate lyase activities | Euglena gracilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
glyoxylate | N-terminal malate synthase-active domain GCE(13-573) of glyoxylate cycle enzyme, at 25°C, pH not specified in the publication | Euglena gracilis | |
17 | - |
glyoxylate | wild type full-length glyoxylate cycle enzyme, at 25°C, pH not specified in the publication | Euglena gracilis |