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Literature summary for 2.3.3.9 extracted from

  • Anstrom, D.M.; Kallio, K.; Remington, S.J.
    Structure of the Escherichia coli malate synthase G:pyruvate:acetyl-coenzyme A abortive ternary complex at 1.95 A resolution (2003), Protein Sci., 12, 1822-1832.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
isoform G in complex with Mg2+, pyruvate, and acetyl-CoA Escherichia coli

Protein Variants

Protein Variants Comment Organism
C617S 88.1% of wild-type activity Escherichia coli
D631N no enzymic activity Escherichia coli
R338K 6.6% of wild-type activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
pyruvate
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
acetyl-CoA wild-type, pH 8.0, 37°C Escherichia coli
0.021
-
glyoxylate wild-type, pH 8.0, 37°C Escherichia coli
0.027
-
glyoxylate mutant R338K, pH 8.0, 37°C Escherichia coli
0.046
-
acetyl-CoA mutant C617S, pH 8.0, 37°C Escherichia coli
0.05
-
glyoxylate mutant C617S, pH 8.0, 37°C Escherichia coli
0.11
-
acetyl-CoA mutant R338K, pH 8.0, 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
isoform G
-

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA mechanism: D631 and R338 act in concert to form the enolate anion of acetyl-CoA in the rate limiting step. C617 is oxidized to cysteine-sulfenic acid Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.39
-
mutant R338K, pH 8.0, 37°C Escherichia coli
31.8
-
mutant C617S, pH 8.0, 37°C Escherichia coli
36.1
-
wild-type, pH 8.0, 37°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + glyoxylate + H2O
-
Escherichia coli (S)-malate + CoA
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.19
-
glyoxylate mutant R338K, pH 8.0, 37°C Escherichia coli
42.4
-
glyoxylate mutant C617S, pH 8.0, 37°C Escherichia coli
48.1
-
glyoxylate wild-type, pH 8.0, 37°C Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1
-
pyruvate mutant C617S, pH 8.0, 37°C Escherichia coli
1
-
pyruvate wild-type, pH 8.0, 37°C Escherichia coli
1.6
-
pyruvate mutant R338K, pH 8.0, 37°C Escherichia coli