Crystallization (Comment) | Organism |
---|---|
isoform G in complex with Mg2+, pyruvate, and acetyl-CoA | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C617S | 88.1% of wild-type activity | Escherichia coli |
D631N | no enzymic activity | Escherichia coli |
R338K | 6.6% of wild-type activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pyruvate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
acetyl-CoA | wild-type, pH 8.0, 37°C | Escherichia coli | |
0.021 | - |
glyoxylate | wild-type, pH 8.0, 37°C | Escherichia coli | |
0.027 | - |
glyoxylate | mutant R338K, pH 8.0, 37°C | Escherichia coli | |
0.046 | - |
acetyl-CoA | mutant C617S, pH 8.0, 37°C | Escherichia coli | |
0.05 | - |
glyoxylate | mutant C617S, pH 8.0, 37°C | Escherichia coli | |
0.11 | - |
acetyl-CoA | mutant R338K, pH 8.0, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
isoform G | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA | mechanism: D631 and R338 act in concert to form the enolate anion of acetyl-CoA in the rate limiting step. C617 is oxidized to cysteine-sulfenic acid | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.39 | - |
mutant R338K, pH 8.0, 37°C | Escherichia coli |
31.8 | - |
mutant C617S, pH 8.0, 37°C | Escherichia coli |
36.1 | - |
wild-type, pH 8.0, 37°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + glyoxylate + H2O | - |
Escherichia coli | (S)-malate + CoA | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.19 | - |
glyoxylate | mutant R338K, pH 8.0, 37°C | Escherichia coli | |
42.4 | - |
glyoxylate | mutant C617S, pH 8.0, 37°C | Escherichia coli | |
48.1 | - |
glyoxylate | wild-type, pH 8.0, 37°C | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
pyruvate | mutant C617S, pH 8.0, 37°C | Escherichia coli | |
1 | - |
pyruvate | wild-type, pH 8.0, 37°C | Escherichia coli | |
1.6 | - |
pyruvate | mutant R338K, pH 8.0, 37°C | Escherichia coli |