BRENDA - Enzyme Database show
show all sequences of 2.3.3.9

Purification and characterization of malate synthase from the glucose-grown wood-rotting basidiomycete Fomitopsis palustris

Munir, E.; Hattori, T.; Shimada, M.; Biosci. Biotechnol. Biochem. 66, 576-581 (2002)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
acetyl-CoA
above 0.02 mM
Fomitopsis palustris
ADP
1 mM, 50% residual activity
Fomitopsis palustris
AMP
1 mM, 54% residual activity
Fomitopsis palustris
ATP
1 mM, 50% residual activity
Fomitopsis palustris
Ca2+
5 mM, 19% residual activity
Fomitopsis palustris
citrate
1 mM, 82% residual activity
Fomitopsis palustris
coenzyme A
0.1 mM, 56% residual activity
Fomitopsis palustris
D-fructose-1,6-bisphosphate
10 mM, 60% residual activity
Fomitopsis palustris
glycolate
1 mM, 77% residual activity
Fomitopsis palustris
Glyoxal
10 mM, 70% residual activity
Fomitopsis palustris
iodoacetate
5 mM, 6% residual activity
Fomitopsis palustris
K+
5 mM, 5% residual activity
Fomitopsis palustris
Mn2+
5 mM, 16% residual activity
Fomitopsis palustris
Na+
5 mM, 10% residual activity
Fomitopsis palustris
oxalate
1 mM, 43% residual activity
Fomitopsis palustris
oxaloacetate
1 mM, 92% residual activity
Fomitopsis palustris
p-chloromercuribenzoate
0.05 mM, 9% residual activity
Fomitopsis palustris
phosphoenolpyruvate
1 mM, 66% residual activity
Fomitopsis palustris
pyruvate
1 mM, 89% residual activity
Fomitopsis palustris
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0022
-
acetyl-CoA
pH 8.0, 30C
Fomitopsis palustris
0.045
-
glyoxalate
pH 8.0, 30C
Fomitopsis palustris
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
maximal enzyme activity at 5-10 mM, Km-value 0.4 mM
Fomitopsis palustris
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
8 * 65000, SDS-PAGE
Fomitopsis palustris
520000
-
gel filtration
Fomitopsis palustris
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Fomitopsis palustris
-
growth on glucose
-
Purification (Commentary)
Commentary
Organism
-
Fomitopsis palustris
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + glyoxylate + H2O
-
658296
Fomitopsis palustris
(S)-malate + CoA
-
-
-
?
additional information
no substrate: oxaloacetate, pyruvate, 2-oxoglutarate, glyoxal
658296
Fomitopsis palustris
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
octamer
8 * 65000, SDS-PAGE
Fomitopsis palustris
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Fomitopsis palustris
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetyl-CoA
above 0.02 mM
Fomitopsis palustris
ADP
1 mM, 50% residual activity
Fomitopsis palustris
AMP
1 mM, 54% residual activity
Fomitopsis palustris
ATP
1 mM, 50% residual activity
Fomitopsis palustris
Ca2+
5 mM, 19% residual activity
Fomitopsis palustris
citrate
1 mM, 82% residual activity
Fomitopsis palustris
coenzyme A
0.1 mM, 56% residual activity
Fomitopsis palustris
D-fructose-1,6-bisphosphate
10 mM, 60% residual activity
Fomitopsis palustris
glycolate
1 mM, 77% residual activity
Fomitopsis palustris
Glyoxal
10 mM, 70% residual activity
Fomitopsis palustris
iodoacetate
5 mM, 6% residual activity
Fomitopsis palustris
K+
5 mM, 5% residual activity
Fomitopsis palustris
Mn2+
5 mM, 16% residual activity
Fomitopsis palustris
Na+
5 mM, 10% residual activity
Fomitopsis palustris
oxalate
1 mM, 43% residual activity
Fomitopsis palustris
oxaloacetate
1 mM, 92% residual activity
Fomitopsis palustris
p-chloromercuribenzoate
0.05 mM, 9% residual activity
Fomitopsis palustris
phosphoenolpyruvate
1 mM, 66% residual activity
Fomitopsis palustris
pyruvate
1 mM, 89% residual activity
Fomitopsis palustris
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0022
-
acetyl-CoA
pH 8.0, 30C
Fomitopsis palustris
0.045
-
glyoxalate
pH 8.0, 30C
Fomitopsis palustris
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
maximal enzyme activity at 5-10 mM, Km-value 0.4 mM
Fomitopsis palustris
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
8 * 65000, SDS-PAGE
Fomitopsis palustris
520000
-
gel filtration
Fomitopsis palustris
Purification (Commentary) (protein specific)
Commentary
Organism
-
Fomitopsis palustris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + glyoxylate + H2O
-
658296
Fomitopsis palustris
(S)-malate + CoA
-
-
-
?
additional information
no substrate: oxaloacetate, pyruvate, 2-oxoglutarate, glyoxal
658296
Fomitopsis palustris
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
octamer
8 * 65000, SDS-PAGE
Fomitopsis palustris
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Fomitopsis palustris
Other publictions for EC 2.3.3.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735447
Costa
Alkaloids as inhibitors of mal ...
Paracoccidioides sp.
Antimicrob. Agents Chemother.
59
5581-5594
2015
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735960
Bauza
Long-range effects in anion-pi ...
Mycobacterium tuberculosis
Chemistry
20
6985-6990
2014
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737048
de Freitas
beta-Carboline alkaloids from ...
Paracoccidioides sp.
Planta Med.
80
1746-1752
2014
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735671
Dahiya
Functional intermediate in the ...
Escherichia coli
Biochemistry
52
4517-4530
2013
-
-
1
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1
1
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1
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735853
de Oliveira
Intermolecular interactions of ...
Paracoccidioides sp., Paracoccidioides sp. ATCC MYA-826
BMC Microbiol.
13
107
2013
-
-
1
-
-
-
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1
2
-
5
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1
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1
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1
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1
1
1
1
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735982
May
A systems chemical biology stu ...
Mycobacterium tuberculosis
Comput. Biol. Chem.
47
167-180
2013
-
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4
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1
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4
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715749
Nakazawa
Characterization of a bifuncti ...
Euglena gracilis
J. Eukaryot. Microbiol.
58
128-133
2011
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-
1
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3
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2
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1
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718909
Quartararo
Kinetic and chemical mechanism ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Biochemistry
50
6879-6887
2011
-
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1
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1
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1
2
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1
1
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719191
Bracken
Crystal structures of a haloph ...
Haloferax volcanii
BMC Struct. Biol.
11
23
2011
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1
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2
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719604
Kumar
Comparative analysis of malate ...
Escherichia coli, Escherichia coli C41, Mycobacterium tuberculosis
Int. J. Biol. Macromol.
49
917-922
2011
-
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2
2
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6
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2
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702487
Maheshwari
Equilibrium and kinetics of th ...
Escherichia coli
Biochimie
92
491-498
2010
-
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1
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1
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715548
Nunn
Metabolism of pentose sugars i ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
J. Biol. Chem.
285
33701-33709
2010
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1
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701846
Sugimoto
Importance of malate synthase ...
Eremothecium gossypii
Appl. Microbiol. Biotechnol.
83
529-539
2009
-
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1
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701928
Anand
Hydrogen peroxide induced oxid ...
Ricinus communis
Arch. Biochem. Biophys.
491
25-31
2009
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702873
Roucourt
Biochemical characterization o ...
Pseudomonas aeruginosa
BMC Biochem.
10
20
2009
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4
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702909
da Silva Neto
The malate synthase of Paracoc ...
Paracoccidioides brasiliensis
BMC Microbiol.
9
272
2009
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705493
Zambuzzi-Carvalho
The malate synthase of Paracoc ...
Paracoccidioides brasiliensis, Paracoccidioides brasiliensis Pb01 / ATCC MYA 826
Med. Mycol.
47
734-744
2009
-
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1
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1
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5
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705567
Dunn
Major roles of isocitrate lyas ...
Bradyrhizobium japonicum, Candida albicans, Escherichia coli, Mycobacterium tuberculosis, Paracoccidioides brasiliensis, Parastagonospora nodorum, Rhizobium leguminosarum, Rhodococcus fascians, Sinorhizobium meliloti, Xanthomonas campestris, Yersinia enterocolitica, Yersinia pestis, Yersinia pseudotuberculosis
Microbiology
155
3166-3175
2009
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2
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1
2
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19
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2
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6
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6
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704727
Grishaev
Refined solution structure of ...
Escherichia coli
J. Biomol. NMR
40
95-106
2008
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706621
Lohman
Atomic resolution structures o ...
Bacillus anthracis, Escherichia coli
Protein Sci.
17
1935-1945
2008
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2
2
2
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5
10
-
2
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8
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2
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1
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2
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10
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2
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2
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680497
Ramirez-Trujillo
Functional characterization of ...
Sinorhizobium meliloti
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5875-5884
2007
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670169
Sakai
Subcellular localization of gl ...
Fomitopsis palustris
Microbiology
152
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2006
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676121
Lattif
The glyoxylate cycle enzyme ac ...
Candida albicans
Mycoses
49
85-90
2006
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658479
Nakazawa
Molecular characterization of ...
Euglena gracilis
Comp. Biochem. Physiol. B
141
445-452
2005
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3
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2
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663463
Lu
Anaerobic induction of isocitr ...
Oryza sativa
Acta Biochim. Biophys. Sin.
37
406-414
2005
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2
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659691
Koh
-
Engineering recombinant Strept ...
Streptomyces coelicolor
J. Microbiol. Biotechnol.
14
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2004
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3
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659963
Solomon
Pathogenicity of Stagonospora ...
Parastagonospora nodorum
Mol. Microbiol.
53
1065-1073
2004
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1
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660310
Nogales
Functional analysis and regula ...
Chlamydomonas reinhardtii
Planta
219
325-331
2004
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4
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638507
Smith
Biochemical and structural stu ...
Mycobacterium tuberculosis
J. Biol. Chem.
278
1735-1743
2003
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1
1
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6
2
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3
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1
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4
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659630
Goh
Thermostable malate synthase o ...
Streptomyces thermovulgaris
J. Ind. Microbiol. Biotechnol.
30
577-581
2003
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1
1
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1
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660456
Anstrom
Structure of the Escherichia c ...
Escherichia coli
Protein Sci.
12
1822-1832
2003
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1
3
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1
6
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4
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6
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3
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1
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3
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638503
Kunze
Targeting of malate synthase 1 ...
Saccharomyces cerevisiae
Eur. J. Biochem.
269
915-922
2002
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1
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3
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638506
Loke
Purification and characterizat ...
Streptomyces clavuligerus, Streptomyces clavuligerus NRRL 3585, Streptomyces coelicolor, Streptomyces coelicolor A3(2)
J. Ind. Microbiol. Biotechnol.
28
239-243
2002
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2
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4
4
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2
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9
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2
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2
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4
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2
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2
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4
-
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2
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2
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658296
Munir
Purification and characterizat ...
Fomitopsis palustris
Biosci. Biotechnol. Biochem.
66
576-581
2002
-
-
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-
19
2
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1
2
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2
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1
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2
1
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19
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2
1
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1
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638504
Watanabe
Purification and characterizat ...
Colwellia maris
Biosci. Biotechnol. Biochem.
65
1095-1103
2001
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3
2
1
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4
2
2
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3
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1
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1
1
1
3
1
1
1
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2
1
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2
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1
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4
2
2
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1
1
1
3
1
1
1
-
2
1
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-
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-
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657618
Goh
Replacement of arginine-171 an ...
Streptomyces coelicolor, Streptomyces coelicolor A3(2)
Appl. Microbiol. Biotechnol.
57
363-367
2001
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2
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5
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1
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3
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2
1
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638502
Howard
Crystal structure of Escherich ...
Escherichia coli
Biochemistry
39
3156-3168
2000
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1
1
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1
1
1
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2
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1
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2
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638505
Mahan
Thermal Dependence of Malate S ...
Gossypium hirsutum, Helianthus annuus
J. Agric. Food Chem.
48
4544-4549
2000
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4
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727500
Serrano
Operation of glyoxylate cycle ...
Haloferax volcanii, Haloferax volcanii DSM 3757
FEBS Lett.
434
13-16
1998
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2
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1
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1
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2
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2
2
2
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1
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1
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4
1
2
1
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2
1
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1
1
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638491
Httener
Gene cloning and sequencing, a ...
Streptomyces arenae
Gene
188
239-246
1997
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6
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1
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1
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1
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2
1
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2
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1
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638492
Mullen
-
Purification and characterizat ...
Pinus taeda
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33
639-648
1995
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1
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1
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1
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1
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638499
Hoppe
Rapid purification of malate s ...
Brassica napus
FEBS Lett.
374
225-227
1995
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2
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1
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1
1
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638495
Reinscheid
Malate synthase from Corynebac ...
Corynebacterium glutamicum
Microbiology
140
3099-3108
1994
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2
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3
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Beeckmans
A specific association between ...
Zea mays
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224
197-201
1994
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Beeckmans
-
Ligand binding on to maize (Ze ...
Zea mays
Biochem. J.
303
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638497
Khan
Purification of the glyoxylate ...
Zea mays
Protein Expr. Purif.
4
519-528
1993
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2
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1
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1
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2
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Henry
-
Salt-mediated interconversions ...
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82
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Bruinenberg
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Purification and some properti ...
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638501
Benvides
Determination of isocitrate ly ...
Petricolaria pholadiformis
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1989
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Yang
-
Phosphorylation of glyoxysomal ...
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2
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3
1
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638493
Mori
-
Purification and characterizat ...
Cucurbita sp.
Plant Cell Physiol.
29
449-460
1988
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1
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3
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1
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1
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1
2
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638471
Trelease
Cottonseed malate synthase ...
Gossypium hirsutum
Plant Physiol.
84
1343-1349
1987
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2
1
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1
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2
1
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638472
Fukawa
-
Purification and some properti ...
Pinus densiflora
Agric. Biol. Chem.
51
1553-1560
1987
1
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1
4
4
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1
4
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1
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1
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2
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1
1
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6
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1
1
4
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4
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1
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1
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4
4
4
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1
4
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1
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2
1
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1
1
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6
-
1
1
4
-
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-
-
-
-
638473
Smith
Glyoxysomal malate synthase of ...
Cucumis sativus
Plant Physiol.
81
762-767
1986
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-
1
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1
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638474
Okada
Purification of peroxisomal ma ...
Candida tropicalis, Candida tropicalis pK 233
Arch. Microbiol.
144
137-141
1986
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-
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1
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3
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4
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3
1
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1
1
2
1
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1
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638475
Durchschlag
Post-irradation inactivation o ...
Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
118
364-370
1984
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-
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1
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1
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638476
Kruse
Oligomerization of malate synt ...
Cucumis sativus
Arch. Biochem. Biophys.
223
629-638
1983
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-
-
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1
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1
1
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1
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2
1
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638477
Kruse
Malate synthase: aggregation, ...
Cucumis sativus
Arch. Biochem. Biophys.
223
618-628
1983
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2
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2
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1
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638478
Miernyk
-
Malate synthase from Gossypium ...
Gossypium hirsutum
Phytochemistry
20
2657-2663
1981
1
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-
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17
2
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4
2
1
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1
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1
1
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2
1
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4
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1
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1
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2
1
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1
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17
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2
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4
2
1
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2
1
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4
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1
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1
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2
1
-
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638479
Durchschlag
Large-scale purification and s ...
Saccharomyces cerevisiae
Eur. J. Biochem.
114
255-262
1981
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-
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4
2
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1
2
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2
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1
1
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1
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1
1
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1
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4
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4
4
2
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1
2
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1
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1
1
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1
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638480
Sundaram
Monomeric malate synthase from ...
Bacillus sp. (in: Bacteria), Escherichia coli, Neurospora crassa
Arch. Biochem. Biophys.
199
515-525
1980
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2
2
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4
2
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4
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1
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1
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5
5
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1
2
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2
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2
2
2
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4
2
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1
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1
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5
5
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1
2
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638481
Chell
Structural basis of the thermo ...
Bacillus licheniformis, Bacillus sp. (in: Bacteria), Escherichia coli, Vogesella indigofera
J. Bacteriol.
135
334-341
1978
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1
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6
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8
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2
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1
3
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4
2
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4
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6
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1
3
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4
2
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4
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638482
Woodcock
Purification and immunchemical ...
Euglena gracilis
Biochem. J.
173
95-101
1978
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2
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2
1
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7
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1
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2
1
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2
1
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1
1
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2
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1
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1
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2
1
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2
1
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1
1
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-
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-
638483
Bowden
Purification and comparative p ...
Ricinus communis
Plant Physiol.
61
259-265
1978
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-
-
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5
2
3
1
2
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3
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1
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3
1
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1
1
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1
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2
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5
2
2
3
1
2
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1
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3
1
-
1
1
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1
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638484
Patel
Particulate isocitrate lyase a ...
Caenorhabditis elegans
Arch. Biochem. Biophys.
183
24-30
1977
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-
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2
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1
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638485
Zipper
Small-angle X-ray studies on m ...
Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
75
394-400
1977
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-
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1
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33133
Chell
Isolation and characterization ...
Geobacillus stearothermophilus
Biochem. Soc. Trans.
3
303-306
1975
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-
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2
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2
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2
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1
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1
1
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1
1
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1
1
1
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2
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2
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1
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1
1
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1
1
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1
1
1
-
-
-
-
-
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-
638487
Schmid
Molecular structure of malate ...
Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
58
419-426
1974
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1
2
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1
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1
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1
1
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638488
Falmagne
-
Purification et caracterisatio ...
Escherichia coli
Eur. J. Biochem.
37
415-424
1973
-
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1
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1
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1
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1
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1
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638489
Begin-Heick
The localization of enzymes of ...
Euglena gracilis, Euglena longa
Biochem. J.
134
607-616
1973
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4
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2
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638490
Dixon
Purification and properties of ...
Pseudomonas putida, Saccharomyces cerevisiae
Biochim. Biophys. Acta
41
217-233
1960
-
-
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3
6
2
-
4
-
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2
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2
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2
2
3
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2
2
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6
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3
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6
6
2
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4
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2
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2
2
3
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2
2
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