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Literature summary for 2.3.3.9 extracted from

  • Dixon, G.H.; Kornberg, H.L.; Lund, P.
    Purification and properties of malate synthetase (1960), Biochim. Biophys. Acta, 41, 217-233.
    View publication on PubMed

General Stability

General Stability Organism
freezing and thawing inactivates Pseudomonas putida
Mg2+ stabilizes Saccharomyces cerevisiae
Mg2+ stabilizes Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
fluoroacetate
-
Pseudomonas putida
fluoroacetate
-
Saccharomyces cerevisiae
glycolate
-
Pseudomonas putida
glycolate
-
Saccharomyces cerevisiae
oxalate
-
Pseudomonas putida
oxalate
-
Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
glyoxylate
-
Pseudomonas putida
0.093
-
glyoxylate
-
Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae
Mg2+ required Pseudomonas putida
Mg2+ Km: 0.5 mM Saccharomyces cerevisiae
Mg2+ Km: 0.5 mM Pseudomonas putida
Mn2+ can partially replace Mg2+ in activation Saccharomyces cerevisiae
Mn2+ can partially replace Mg2+ in activation Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Saccharomyces cerevisiae
additional information
-
-
Pseudomonas putida

Storage Stability

Storage Stability Organism
2°C, stable for at least a month Saccharomyces cerevisiae
2°C, stable for at least a month Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glyoxylate + acetyl-CoA + H2O
-
Saccharomyces cerevisiae (S)-malate + CoA
-
ir
glyoxylate + acetyl-CoA + H2O
-
Pseudomonas putida (S)-malate + CoA
-
ir
glyoxylate + fluoroacetyl-CoA + H2O
-
Pseudomonas putida 2-oxo-3-fluoro-butanedioic acid + CoA
-
ir

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Saccharomyces cerevisiae
additional information
-
additional information
-
Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Saccharomyces cerevisiae
8.5
-
-
Pseudomonas putida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.019
-
oxalate
-
Saccharomyces cerevisiae
0.019
-
oxalate
-
Pseudomonas putida
0.246
-
fluoroacetate
-
Saccharomyces cerevisiae
0.246
-
fluoroacetate
-
Pseudomonas putida
0.308
-
glycolate
-
Saccharomyces cerevisiae
0.308
-
glycolate
-
Pseudomonas putida