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Literature summary for 2.3.3.9 extracted from

  • Miernyk, J.A.; Trelease, R.N.
    Malate synthase from Gossypium hirsutum (1981), Phytochemistry, 20, 2657-2663.
No PubMed abstract available
Search for more literature for 2.3.3.9

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 activation Gossypium hirsutum

Inhibitors

Inhibitors Comment Organism Structure
acetaldehyde weak Gossypium hirsutum
ADP
-
 Gossypium hirsutum
ATP
-
 Gossypium hirsutum
Butanedione slight inhibition at high concentrations Gossypium hirsutum
chloroacetyl-CoA
-
 Gossypium hirsutum
deamino-acetyl-CoA
-
 Gossypium hirsutum
deoxycholate
-
 Gossypium hirsutum
dephosphoacetyl-CoA
-
 Gossypium hirsutum
diethyl dicarbonate inhibition prevented by preincubation with acetyl-CoA Gossypium hirsutum
glycolate
-
 Gossypium hirsutum
methylglyoxal
-
 Gossypium hirsutum
oxalate
-
 Gossypium hirsutum
oxamate
-
 Gossypium hirsutum
pyridoxal-5'-phosphate preincubation with glyoxylate but not acetyl-CoA prevents inhibition Gossypium hirsutum
pyruvate weak Gossypium hirsutum
S-acetonyl-CoA
-
 Gossypium hirsutum
S-ethyl-CoA
-
 Gossypium hirsutum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 acetyl-CoA
-
 Gossypium hirsutum
0.052
-
 glyoxylate
-
 Gossypium hirsutum

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ about 30% of the activation with Mg2+ Gossypium hirsutum
Co2+ about 30% of the activation with Mg2+ Gossypium hirsutum
Mg2+ Km: 0.59 mM Gossypium hirsutum
Mg2+ absolute requirement for divalent metal ion, best fulfilled by Mg2+ Gossypium hirsutum
Mn2+ about 30% of the activation with Mg2+ Gossypium hirsutum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
730000
-
 gel filtration Gossypium hirsutum
750000
-
 sucrose density gradient centrifugation Gossypium hirsutum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glyoxylate + acetyl-CoA + H2O Gossypium hirsutum enzyme specifically involved in glyoxalate cycle metabolism (S)-malate + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Gossypium hirsutum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gossypium hirsutum

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA sequential bireactant mechanism Gossypium hirsutum
a

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon of dark-germinated seeds Gossypium hirsutum
-
seed
-
 Gossypium hirsutum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Gossypium hirsutum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glyoxylate + acetyl-CoA + H2O
-
 Gossypium hirsutum (S)-malate + CoA
-
 ir
glyoxylate + acetyl-CoA + H2O enzyme specifically involved in glyoxalate cycle metabolism Gossypium hirsutum (S)-malate + CoA
-
 ?
glyoxylate + butyryl-CoA + H2O
-
 Gossypium hirsutum 2-oxohexanedioic acid + CoA
-
 ir
glyoxylate + propionyl-CoA + H2O
-
 Gossypium hirsutum 2-oxopentanedioic acid + CoA
-
 ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Gossypium hirsutum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 2 min, 84% loss of activity Gossypium hirsutum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 Tris/HCl buffer Gossypium hirsutum
8.2
-
 phosphate-citrate-borate buffer or MOPS-KOH buffer Gossypium hirsutum

pH Range

pH Minimum pH Maximum Comment Organism
6.8 9.7 pH 6.8 and 9.7: about 50% of maximal activity, phosphate-citrate-borate buffer Gossypium hirsutum