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Literature summary for 2.3.3.9 extracted from
- Isolation and characterization of isocitrate lyase and malate synthase from Bacillus stearothermophilus (1975), Biochem. Soc. Trans., 3, 303-306.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
acetyl-CoA | - |
Geobacillus stearothermophilus |  |
| 0.088 | - |
acetyl-CoA | - |
Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
1 * 58000, SDS-PAGE | Geobacillus stearothermophilus |
| 62000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:acetate-grown cell | - |
Geobacillus stearothermophilus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + acetyl-CoA + H2O | - |
Geobacillus stearothermophilus | (S)-malate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 58000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
less than 10% loss of activity after 2 h at pH 8 | Geobacillus stearothermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26.7 | - |
acetyl-CoA | - |
Geobacillus stearothermophilus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
- |
Geobacillus stearothermophilus |
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Release 2023.1 (January 2023)
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