Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.3.8 extracted from

  • Ranganathan, N.S.; Srere, P.A.; Linn, T.C.
    Comparison of phospho- and dephospho-ATP citrate lyase (1980), Arch. Biochem. Biophys., 204, 52-58.
    View publication on PubMed
Search for more literature for 2.3.3.8

Inhibitors

Inhibitors Comment Organism Structure
(4S)-hydroxycitrate
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Rattus norvegicus
0.054
-
 ATP phosphoenzyme in Hepes buffer, pH 7.6 Rattus norvegicus
0.208
-
 ATP dephosphoenzyme in Tris-HCl buffer, pH 8.7 Rattus norvegicus
0.222
-
 ATP phosphoenzyme in Tris-HCl buffer, pH 8.7 Rattus norvegicus
0.292
-
 ATP dephosphoenzyme in Hepes buffer, pH 7.6 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion the enzyme tends to associate with the mitochondria Rattus norvegicus 5739
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification phosphoprotein Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + citrate + CoA
-
 Rattus norvegicus ADP + phosphate + acetyl-CoA + oxaloacetate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 30 min, protein concentration of 0.25 mg/ml, phosphorylated and dephosphorylated form of enzyme, stable Rattus norvegicus
45
-
 10 min, protein concentration of 0.16 mg/ml, phosphorylated and dephosphorylated form of enzyme, 50% loss of activity Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00005
-
 (4S)-hydroxycitrate phospho-form of enzyme Rattus norvegicus
0.00007
-
 (4S)-hydroxycitrate dephospho-form of enzyme Rattus norvegicus