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Literature summary for 2.3.3.8 extracted from

  • Boulton, C.A.; Ratledge, C.
    Partial purification and some properties of ATP:citrate lyase from the oleaginous yeast Lipomyces starkeyi (1983), J. Gen. Microbiol., 129, 2863-2869.
No PubMed abstract available
Search for more literature for 2.3.3.8

General Stability

General Stability Organism
ATP and citrate stabilize Lipomyces starkeyi

Inhibitors

Inhibitors Comment Organism Structure
lauroyl-CoA 10 mM, 52% inhibition Lipomyces starkeyi
myristoyl-CoA 10 mM, 5% inhibition Lipomyces starkeyi
oleoyl-CoA 10 mM, 21% inhibition Lipomyces starkeyi
palmitoyl-CoA 10 mM, 5% inhibition Lipomyces starkeyi
stearoyl-CoA 10 mM, 45% inhibition Lipomyces starkeyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 citrate
-
 Lipomyces starkeyi
0.135
-
 ATP
-
 Lipomyces starkeyi

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 37.1% of the activation with Mg2+ at 10 mM Lipomyces starkeyi
Mg2+ required Lipomyces starkeyi
Mn2+ 22.6% of the activation with Mg2+ at 10 mM Lipomyces starkeyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
510000
-
 gel filtration Lipomyces starkeyi

Organism

Organism UniProt Comment Textmining
Lipomyces starkeyi
-
 CBS 1809
-
Lipomyces starkeyi CBS 1809
-
 CBS 1809
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lipomyces starkeyi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.922
-
-
 Lipomyces starkeyi

Storage Stability

Storage Stability Organism
4°C, 50 mM Tris-HCl, pH 7.5, 1 mM MgCl2, 10 mM 2-mercaptoethanol, 83% loss of activity after 48 h. 52% loss of activity in presence of 1 mM ATP. 30% loss of activity in presence of 20 mM citrate, 9% loss of activity in presence of 1 mM ATP and 20 mM citrate Lipomyces starkeyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + citrate + CoA specific for ATP Lipomyces starkeyi ADP + phosphate + acetyl-CoA + oxaloacetate
-
 ?
ATP + citrate + CoA specific for ATP Lipomyces starkeyi CBS 1809 ADP + phosphate + acetyl-CoA + oxaloacetate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2 8.6
-
 Lipomyces starkeyi

pH Range

pH Minimum pH Maximum Comment Organism
7.3 9.5 about 50% of maximal activity at pH 7.3 and at pH 9.5 Lipomyces starkeyi