BRENDA - Enzyme Database show
show all sequences of 2.3.3.6

A study of acetyl-CoA condensation with alpha-keto acids

Strassman, M.; Ceci, L.N.; Arch. Biochem. Biophys. 119, 420-428 (1967)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
(NH4)2SO4
maximum of activity: about 45% (NH4)2SO4 and decrease in activity at higher concentrations
Saccharomyces cerevisiae
Inhibitors
Inhibitors
Commentary
Organism
Structure
cysteine
-
Saccharomyces cerevisiae
Mg2+
-
Saccharomyces cerevisiae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Reaction
Reaction
Commentary
Organism
acetyl-CoA + H2O + 2-oxobutanoate = (R)-2-ethylmalate + CoA
also acts on (R)-2-(n-propyl)-malate
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + H2O + 2-ketovalerate
-
488178
Saccharomyces cerevisiae
(R)-2-(n-propyl)-malate + CoA
-
488178
Saccharomyces cerevisiae
?
acetyl-CoA + H2O + 2-oxobutanoate
-
488178
Saccharomyces cerevisiae
(R)-2-ethylmalate + CoA
-
488178
Saccharomyces cerevisiae
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
heating in boiling water for 1 min results in a total loss of activity
Saccharomyces cerevisiae
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
(NH4)2SO4
maximum of activity: about 45% (NH4)2SO4 and decrease in activity at higher concentrations
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cysteine
-
Saccharomyces cerevisiae
Mg2+
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + H2O + 2-ketovalerate
-
488178
Saccharomyces cerevisiae
(R)-2-(n-propyl)-malate + CoA
-
488178
Saccharomyces cerevisiae
?
acetyl-CoA + H2O + 2-oxobutanoate
-
488178
Saccharomyces cerevisiae
(R)-2-ethylmalate + CoA
-
488178
Saccharomyces cerevisiae
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
heating in boiling water for 1 min results in a total loss of activity
Saccharomyces cerevisiae
Other publictions for EC 2.3.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
488179
Rabin
Metabolism of ethylmalic acids ...
Pseudomonas aeruginosa
Biochem. J.
7
377-389
1968
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488178
Strassman
A study of acetyl-CoA condensa ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
119
420-428
1967
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