Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli with polyhistidine tags | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | Bacillus subtilis | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase | citrate + CoA | - |
? | |
acetyl-CoA + H2O + oxaloacetate | Bacillus subtilis 168 | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase | citrate + CoA | - |
? | |
propanoyl-CoA + H2O + oxaloacetate | Bacillus subtilis | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) | (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? | |
propanoyl-CoA + H2O + oxaloacetate | Bacillus subtilis 168 | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) | (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P45858 | - |
- |
Bacillus subtilis 168 | P45858 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase | Bacillus subtilis | citrate + CoA | - |
? | |
acetyl-CoA + H2O + oxaloacetate | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) | Bacillus subtilis | citrate + CoA | - |
? | |
acetyl-CoA + H2O + oxaloacetate | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase | Bacillus subtilis 168 | citrate + CoA | - |
? | |
acetyl-CoA + H2O + oxaloacetate | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) | Bacillus subtilis 168 | citrate + CoA | - |
? | |
propanoyl-CoA + H2O + oxaloacetate | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase | Bacillus subtilis | (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? | |
propanoyl-CoA + H2O + oxaloacetate | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) | Bacillus subtilis | (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? | |
propanoyl-CoA + H2O + oxaloacetate | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase | Bacillus subtilis 168 | (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? | |
propanoyl-CoA + H2O + oxaloacetate | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) | Bacillus subtilis 168 | (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
citrate synthase/2-methylcitrate synthase | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase | Bacillus subtilis |
mmgD | bifunctional citrate/2-methylcitrate synthase | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the methylcitric acid cycle | Bacillus subtilis |