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Literature summary for 2.3.3.5 extracted from

  • Reddick, J.J.; Sirkisoon, S.; Dahal, R.A.; Hardesty, G.; Hage, N.E.; Booth, W.T.; Quattlebaum, A.L.; Mills, S.N.; Meadows, V.G.; Adams, S.L.H.; Doyle, J.S.; Kiel, B.E.
    First biochemical characterization of a methylcitric acid cycle from Bacillus subtilis strain 168 (2017), Biochemistry, 56, 5698-5711 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli with polyhistidine tags Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate Bacillus subtilis bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase citrate + CoA
-
?
acetyl-CoA + H2O + oxaloacetate Bacillus subtilis 168 bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase citrate + CoA
-
?
propanoyl-CoA + H2O + oxaloacetate Bacillus subtilis bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?
propanoyl-CoA + H2O + oxaloacetate Bacillus subtilis 168 bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P45858
-
-
Bacillus subtilis 168 P45858
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase Bacillus subtilis citrate + CoA
-
?
acetyl-CoA + H2O + oxaloacetate bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) Bacillus subtilis citrate + CoA
-
?
acetyl-CoA + H2O + oxaloacetate bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase Bacillus subtilis 168 citrate + CoA
-
?
acetyl-CoA + H2O + oxaloacetate bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) Bacillus subtilis 168 citrate + CoA
-
?
propanoyl-CoA + H2O + oxaloacetate bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase Bacillus subtilis (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?
propanoyl-CoA + H2O + oxaloacetate bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) Bacillus subtilis (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?
propanoyl-CoA + H2O + oxaloacetate bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase Bacillus subtilis 168 (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?
propanoyl-CoA + H2O + oxaloacetate bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) Bacillus subtilis 168 (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?

Synonyms

Synonyms Comment Organism
citrate synthase/2-methylcitrate synthase bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase Bacillus subtilis
mmgD bifunctional citrate/2-methylcitrate synthase Bacillus subtilis

General Information

General Information Comment Organism
metabolism the enzyme is involved in the methylcitric acid cycle Bacillus subtilis