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Literature summary for 2.3.3.5 extracted from

  • Kobayashi, K.; Hattori, T.; Honda, Y.; Kirimura, K.
    Gene identification and functional analysis of methylcitrate synthase in citric acid-producing Aspergillus niger WU-2223L (2013), Biosci. Biotechnol. Biochem., 77, 1492-1498.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Aspergillus niger 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51300
-
x * 51300, SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
propanoyl-CoA + H2O + oxaloacetate Aspergillus niger
-
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?
propanoyl-CoA + H2O + oxaloacetate Aspergillus niger WU-2223L
-
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger WU-2223L
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme also exhibits citrate synthase activity Aspergillus niger ?
-
?
additional information the enzyme also exhibits citrate synthase activity Aspergillus niger WU-2223L ?
-
?
propanoyl-CoA + H2O + oxaloacetate
-
Aspergillus niger (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?
propanoyl-CoA + H2O + oxaloacetate
-
Aspergillus niger WU-2223L (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?

Subunits

Subunits Comment Organism
? x * 51300, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
MCS
-
Aspergillus niger
Methylcitrate synthase
-
Aspergillus niger

General Information

General Information Comment Organism
metabolism the enzyme is essential in propionic acid metabolism Aspergillus niger