BRENDA - Enzyme Database show
show all sequences of 2.3.3.5

Methylcitrate synthase from Aspergillus fumigatus is essential for manifestation of invasive aspergillosis

Ibrahim-Granet, O.; Dubourdeau, M.; Latgé, J.; Ave, P.; Huerre, M.; Brakhage, A.; Brock, M.; Cell. Microbiol. 10, 134-148 (2008)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
invasive aspergillosis is a life-threatening disease mainly caused by the fungus Aspergillus fumigatus, blocking of methylcitrate synthase abrogates fungal growth and provides a suitable target for new antifungals
Aspergillus fumigatus
Cloned(Commentary)
Commentary
Organism
into the pCR2.1 and pUChph1 vectors
Aspergillus fumigatus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
propionyl-CoA + H2O + oxaloacetate
Aspergillus fumigatus
-
(2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus fumigatus
-
wild-type strain CBS 144.89, methylcitrate synthase mutant DELTAmcsA, complemented methylcitrate synthase mutant 11/12 B1
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.003
-
wild-type strain, carbon source glucose
Aspergillus fumigatus
0.004
-
complemented methylcitrate synthase mutant 11/12 B1, carbon source glucose
Aspergillus fumigatus
0.011
-
wild-type strain, carbon source olive oil
Aspergillus fumigatus
0.029
-
complemented methylcitrate synthase mutant 11/12 B1, carbon source olive oil
Aspergillus fumigatus
0.085
-
wild-type strain, carbon source peptone
Aspergillus fumigatus
0.165
-
complemented methylcitrate synthase mutant 11/12 B1, carbon source peptone
Aspergillus fumigatus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + H2O + oxaloacetate
-
703115
Aspergillus fumigatus
citrate + CoA
-
-
-
?
propionyl-CoA + H2O + oxaloacetate
-
703115
Aspergillus fumigatus
(2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
CoA
-
Aspergillus fumigatus
Application (protein specific)
Application
Commentary
Organism
medicine
invasive aspergillosis is a life-threatening disease mainly caused by the fungus Aspergillus fumigatus, blocking of methylcitrate synthase abrogates fungal growth and provides a suitable target for new antifungals
Aspergillus fumigatus
Cloned(Commentary) (protein specific)
Commentary
Organism
into the pCR2.1 and pUChph1 vectors
Aspergillus fumigatus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
CoA
-
Aspergillus fumigatus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
propionyl-CoA + H2O + oxaloacetate
Aspergillus fumigatus
-
(2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.003
-
wild-type strain, carbon source glucose
Aspergillus fumigatus
0.004
-
complemented methylcitrate synthase mutant 11/12 B1, carbon source glucose
Aspergillus fumigatus
0.011
-
wild-type strain, carbon source olive oil
Aspergillus fumigatus
0.029
-
complemented methylcitrate synthase mutant 11/12 B1, carbon source olive oil
Aspergillus fumigatus
0.085
-
wild-type strain, carbon source peptone
Aspergillus fumigatus
0.165
-
complemented methylcitrate synthase mutant 11/12 B1, carbon source peptone
Aspergillus fumigatus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + H2O + oxaloacetate
-
703115
Aspergillus fumigatus
citrate + CoA
-
-
-
?
propionyl-CoA + H2O + oxaloacetate
-
703115
Aspergillus fumigatus
(2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
methylcitrate cycle
Aspergillus fumigatus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
methylcitrate cycle
Aspergillus fumigatus
Other publictions for EC 2.3.3.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735578
Eniyan
Cloning, expression, purificat ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Asian Pac. J. Trop. Med.
8
19-23
2015
-
-
1
-
-
-
-
-
1
-
2
2
-
161
-
-
1
-
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2
1
-
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-
-
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-
1
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1
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-
-
-
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-
1
-
2
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
735795
Kobayashi
Gene identification and functi ...
Aspergillus niger, Aspergillus niger WU-2223L
Biosci. Biotechnol. Biochem.
77
1492-1498
2013
-
-
1
-
-
-
-
-
1
-
1
2
-
5
-
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1
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4
1
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1
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1
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1
2
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1
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-
4
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
720336
Chittori
Crystal structure of Salmonell ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Struct. Biol.
174
58-68
2011
-
-
-
-
-
-
2
4
-
-
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4
-
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3
1
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1
2
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2
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4
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-
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3
1
-
-
1
2
-
-
-
-
-
-
-
-
2
2
704332
Rocco
In Salmonella enterica, 2-meth ...
Salmonella enterica, Salmonella enterica JE2170
J. Bacteriol.
192
771-778
2010
-
-
-
-
-
-
-
-
1
-
-
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5
-
-
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2
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1
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2
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-
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-
-
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-
705576
Domin
Methylcitrate cycle activation ...
Fusarium solani, Fusarium verticillioides
Microbiology
155
3903-3912
2009
-
-
2
-
-
-
-
8
-
-
2
2
-
9
-
-
2
-
-
-
4
-
4
-
-
2
-
4
-
2
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
8
-
-
2
2
-
-
-
2
-
-
4
-
4
-
-
2
-
4
-
2
-
-
-
2
2
-
-
-
703115
Ibrahim-Granet
Methylcitrate synthase from As ...
Aspergillus fumigatus
Cell. Microbiol.
10
134-148
2008
-
1
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
6
-
2
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1
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1
1
1
-
-
-
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-
1
-
-
-
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6
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
705770
Fleck
Characterization of an acyl-Co ...
Aspergillus nidulans
Mol. Microbiol.
68
642-656
2008
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
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1
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-
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-
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-
1
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
671529
Ewering
Occurrence and expression of t ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Appl. Microbiol. Biotechnol.
71
80-89
2006
-
-
1
-
-
-
-
2
-
-
1
-
-
5
-
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8
1
1
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1
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1
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1
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2
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1
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-
8
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
675795
Ewering
Metabolic engineering of strai ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Metab. Eng.
8
587-602
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
46
-
-
-
-
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1
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-
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-
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-
-
-
-
-
-
-
-
-
661700
Maerker
Methylcitrate synthase from As ...
Aspergillus fumigatus
FEBS J.
272
3615-3630
2005
-
-
-
-
1
-
2
5
1
-
2
1
-
5
-
-
1
-
-
2
2
-
3
1
1
-
2
-
1
-
1
-
1
2
-
-
-
-
-
-
1
-
-
2
1
5
1
-
2
1
-
-
-
1
-
2
2
-
3
1
1
-
2
-
1
-
1
2
-
-
-
-
-
-
488087
Karlsson
Rhodothermus marinus: a thermo ...
Escherichia coli, Pseudomonas aeruginosa, Rhodothermus marinus
Extremophiles
6
51-56
2002
-
-
2
-
-
-
-
4
-
-
-
3
-
3
-
-
1
-
-
-
1
-
8
2
1
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
4
-
-
-
3
-
-
-
1
-
-
1
-
8
2
1
1
1
-
-
-
-
-
-
-
-
-
-
-
488174
Braemer
Identification of the 2-methyl ...
Aspergillus nidulans, Burkholderia cepacia, Burkholderia cepacia contig261, Cupriavidus metallidurans, Escherichia coli, Escherichia coli O157:H17, Legionella pneumophila, Neisseria meningitidis, Paraburkholderia sacchari, Paraburkholderia sacchari IPT101, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas fluorescens contig267, Pseudomonas putida, Pseudomonas putida contig10768, Pseudomonas putida KT 2240, Pseudomonas syringae, Pseudomonas syringae contig5376, Pyrococcus furiosus, Salmonella enterica, Shewanella putrefaciens, Shewanella putrefaciens contig93, Vibrio cholerae
Appl. Environ. Microbiol.
68
271-279
2002
-
-
1
-
-
-
-
-
-
-
1
33
-
74
-
-
-
-
-
-
-
-
41
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
33
-
-
-
-
-
-
-
-
41
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488175
Brock
Oxidation of propionate to pyr ...
Cupriavidus metallidurans, Escherichia coli, Salmonella enterica, Yarrowia lipolytica
Eur. J. Biochem.
269
6184-6194
2002
-
-
2
-
-
-
-
-
-
-
1
5
-
7
-
-
1
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
5
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488176
Claes
Identification of two prpDBC g ...
Aspergillus nidulans, Corynebacterium glutamicum, Cupriavidus metallidurans, Escherichia coli, Paraburkholderia sacchari, Pseudomonas aeruginosa, Salmonella enterica, Yarrowia lipolytica
J. Bacteriol.
184
2728-2739
2002
-
-
1
-
-
-
-
-
-
-
2
12
-
15
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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-
2
12
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488173
Braemer
The methylcitric acid pathway ...
Aspergillus nidulans, Cupriavidus metallidurans, Escherichia coli, Neisseria meningitidis, Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas putida KT 2240, Salmonella enterica, Vibrio cholerae
Microbiology
147
2203-2214
2001
-
1
1
-
-
-
-
-
-
-
1
9
-
49
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
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-
1
1
-
-
-
-
-
-
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1
9
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488089
Brock
Methylcitrate synthase from As ...
Aspergillus nidulans
Mol. Microbiol.
35
961-973
2000
-
-
1
-
1
-
2
3
2
1
3
1
-
3
-
1
1
-
-
1
4
-
3
1
1
1
-
2
1
1
-
-
-
-
-
-
-
1
-
-
1
-
-
2
-
3
2
1
3
1
-
-
1
1
-
1
4
-
3
1
1
1
-
2
1
1
-
-
-
-
-
-
-
-
488172
Gerike
-
Citrate synthase and 2-methylc ...
Arthrobacter sp., Escherichia coli, Escherichia coli JM109, no activity in Sus scrofa, Pyrococcus furiosus, Saccharomyces cerevisiae, Salmonella enterica, Thermoplasma acidophilum, Yarrowia lipolytica
Microbiology
144
929-935
1998
-
-
1
-
-
-
-
5
-
-
-
8
-
38
-
-
3
-
-
-
-
-
10
-
-
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1
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5
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8
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3
-
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-
-
10
-
-
-
-
-
-
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-
-
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-
-
-
-
-
488171
Textor
Propionate oxidation in Escher ...
Escherichia coli, Saccharomyces cerevisiae, Salmonella enterica, Yarrowia lipolytica
Arch. Microbiol.
168
428-436
1997
-
-
-
-
-
-
-
1
-
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1
6
-
10
-
-
1
-
-
-
1
-
6
-
1
-
-
-
1
-
-
-
-
-
-
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-
-
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-
-
-
-
-
-
1
-
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1
6
-
-
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1
-
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1
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
488170
Pronk
-
Propionate metabolism in Sacch ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae CBS 8066, Yarrowia lipolytica
Microbiology
140
717-722
1994
-
-
-
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-
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-
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3
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4
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5
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3
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5
-
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-
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-
5348
Miyakoshi
-
Distribution of the methylcitr ...
Aspergillus niger, Candida gropengiesseri, Citeromyces matritensis, Cryptococcus neoformans, Cutaneotrichosporon curvatus, Cyberlindnera saturnus, Cyberlindnera saturnus IAM 4094, Debaryomyces hansenii, Diutina catenulata, Kluyveromyces marxianus, Lipomyces starkeyi, Metschnikowia pulcherrima, Mucor rouxianus, Neurospora crassa, no activity in Candida rugosa, Ogataea angusta, Priceomyces haplophilus, Pseudomonas aeruginosa, Rhizopus microsporus var. chinensis, Rhizopus microsporus var. chinensis IAM 6003, Saccharomyces cerevisiae, Saccharomyces cerevisiae SANK 50182, Saccharomycopsis fibuligera, Schwanniomyces polymorphus, Starmerella magnoliae, Torulaspora delbrueckii, Ustilago crus-galli, Ustilago crus-galli F-B-6, Ustilago utriculosa, Ustilago utriculosa F-B-5, Vanrija humicola, Wickerhamiella pararugosa, Wickerhamomyces anomalus, Yarrowia lipolytica
Agric. Biol. Chem.
51
2381-2387
1987
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36
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34
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36
-
43
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-
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-
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36
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36
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43
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488168
Watson
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Pseudomonas aeruginosa contain ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa PAO, Pseudomonas citronellolis, Pseudomonas putida, Yarrowia lipolytica
Curr. Microbiol.
8
17-21
1983
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5
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11
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1
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-
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14
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5
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1
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14
-
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5344
Uchiyama
Subcellular localization of th ...
Yarrowia lipolytica
Eur. J. Biochem.
125
523-527
1982
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1
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1
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1
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4
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3
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1
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1
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4
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3
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488166
Uchiyama
-
Properties of methylcitrate sy ...
Escherichia coli, Yarrowia lipolytica
Agric. Biol. Chem.
40
1411-1418
1976
1
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9
5
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3
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3
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2
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1
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2
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9
-
1
1
1
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1
1
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1
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9
-
5
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3
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3
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1
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2
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9
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1
1
1
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1
1
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488165
Tabuchi
-
Methylcitrate condensing and m ...
Yarrowia lipolytica
Agric. Biol. Chem.
39
2035-2042
1975
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1
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1
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1
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5
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1
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1
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1
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5
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1
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