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Literature summary for 2.3.3.5 extracted from
- Methylcitrate synthase from Aspergillus fumigatus. Propionyl-CoA affects polyketide synthesis, growth and morphology of conidia (2005), FEBS J., 272, 3615-3630.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of an enzyme deletion mutant strain, which is unable to grow on propionate as sole carbon source, phenotype and growth behaviour analysis, accumulation of propionyl-CoA, morphology of conidia, deletion leads to an attenuation of virulence, overview | Aspergillus fumigatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | competitive to CoA | Aspergillus fumigatus |  |
| propionyl-CoA | competitive to CoA | Aspergillus fumigatus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0019 | - |
propanoyl-CoA | pH 8.0, 25°C, purified native enzyme | Aspergillus fumigatus | |
| 0.0026 | - |
acetyl-CoA | pH 8.0, 25°C, purified native enzyme | Aspergillus fumigatus | |
| 0.0027 | - |
oxaloacetate | pH 8.0, 25°C, purified native enzyme | Aspergillus fumigatus | |
| 0.0085 | - |
CoA | pH 8.0, 25°C, purified native enzyme, in absence of propionyl-CoA | Aspergillus fumigatus | |
| 0.0325 | - |
CoA | pH 8.0, 25°C, purified native enzyme, in presence of propionyl-CoA | Aspergillus fumigatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | enzyme possesses a mitochondrial signal import sequence | Aspergillus fumigatus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, native enzyme, SDS-PAGE, x * 514100, sequence calculation | Aspergillus fumigatus |
| 514100 | - |
x * 45000, native enzyme, SDS-PAGE, x * 514100, sequence calculation | Aspergillus fumigatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Aspergillus fumigatus | key enzyme of the methylcitrate cycle and required for fungal propionate degradation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | Q50I20 | opportunistic human pathogen, strain CEA17, strain ATCC 46654 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 136fold to homogeneity | Aspergillus fumigatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Aspergillus fumigatus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 17.7 | - |
purified native enzyme, with oxaloacetate and propionyl-CoA | Aspergillus fumigatus |
| 48 | - |
purified native enzyme, with oxaloacetate and acetyl-CoA | Aspergillus fumigatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + H2O + oxaloacetate | the enzyme also performs the citrate synthase reaction, EC 2.3.3.1 | Aspergillus fumigatus | citrate + CoA | - |
? | |
| additional information | key enzyme of the methylcitrate cycle and required for fungal propionate degradation | Aspergillus fumigatus | ? | - |
? | |
| propanoyl-CoA + H2O + oxaloacetate | reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate | Aspergillus fumigatus | (2S,3S)-2-methylcitrate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, native enzyme, SDS-PAGE, x * 514100, sequence calculation | Aspergillus fumigatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Methylcitrate synthase | - |
Aspergillus fumigatus |
| MscA | - |
Aspergillus fumigatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | - |
Aspergillus fumigatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
3 h, stable up to | Aspergillus fumigatus |
| 60 | - |
half-life 11 min | Aspergillus fumigatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Aspergillus fumigatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | 3 h, stable | Aspergillus fumigatus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.053 | - |
propionyl-CoA | versus CoA | Aspergillus fumigatus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus fumigatus | 2 peaks | - |
6.9 |
| Aspergillus fumigatus | 2 peaks | - |
9 |
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