BRENDA - Enzyme Database show
show all sequences of 2.3.3.5

Methylcitrate synthase from Aspergillus fumigatus. Propionyl-CoA affects polyketide synthesis, growth and morphology of conidia

Maerker, C.; Rohde, M.; Brakhage, A.A.; Brock, M.; FEBS J. 272, 3615-3630 (2005)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of an enzyme deletion mutant strain, which is unable to grow on propionate as sole carbon source, phenotype and growth behaviour analysis, accumulation of propionyl-CoA, morphology of conidia, deletion leads to an attenuation of virulence, overview
Aspergillus fumigatus
Inhibitors
Inhibitors
Commentary
Organism
Structure
acetyl-CoA
competitive to CoA
Aspergillus fumigatus
propionyl-CoA
competitive to CoA
Aspergillus fumigatus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0019
-
propanoyl-CoA
pH 8.0, 25°C, purified native enzyme
Aspergillus fumigatus
0.0026
-
acetyl-CoA
pH 8.0, 25°C, purified native enzyme
Aspergillus fumigatus
0.0027
-
oxaloacetate
pH 8.0, 25°C, purified native enzyme
Aspergillus fumigatus
0.0085
-
CoA
pH 8.0, 25°C, purified native enzyme, in absence of propionyl-CoA
Aspergillus fumigatus
0.0325
-
CoA
pH 8.0, 25°C, purified native enzyme, in presence of propionyl-CoA
Aspergillus fumigatus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
enzyme possesses a mitochondrial signal import sequence
Aspergillus fumigatus
5739
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
x * 45000, native enzyme, SDS-PAGE, x * 514100, sequence calculation
Aspergillus fumigatus
514100
-
x * 45000, native enzyme, SDS-PAGE, x * 514100, sequence calculation
Aspergillus fumigatus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Aspergillus fumigatus
key enzyme of the methylcitrate cycle and required for fungal propionate degradation
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus fumigatus
Q50I20
opportunistic human pathogen, strain CEA17, strain ATCC 46654
-
Purification (Commentary)
Commentary
Organism
native enzyme 136fold to homogeneity
Aspergillus fumigatus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus fumigatus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
17.7
-
purified native enzyme, with oxaloacetate and propionyl-CoA
Aspergillus fumigatus
48
-
purified native enzyme, with oxaloacetate and acetyl-CoA
Aspergillus fumigatus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + H2O + oxaloacetate
the enzyme also performs the citrate synthase reaction, EC 2.3.3.1
661700
Aspergillus fumigatus
citrate + CoA
-
-
-
?
additional information
key enzyme of the methylcitrate cycle and required for fungal propionate degradation
661700
Aspergillus fumigatus
?
-
-
-
-
propanoyl-CoA + H2O + oxaloacetate
reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate
661700
Aspergillus fumigatus
(2S,3S)-2-methylcitrate + CoA
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 45000, native enzyme, SDS-PAGE, x * 514100, sequence calculation
Aspergillus fumigatus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
60
-
Aspergillus fumigatus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
3 h, stable up to
Aspergillus fumigatus
60
-
half-life 11 min
Aspergillus fumigatus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Aspergillus fumigatus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
9
3 h, stable
Aspergillus fumigatus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.053
-
propionyl-CoA
versus CoA
Aspergillus fumigatus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Aspergillus fumigatus
2 peaks
-
6.9
Aspergillus fumigatus
2 peaks
-
9
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of an enzyme deletion mutant strain, which is unable to grow on propionate as sole carbon source, phenotype and growth behaviour analysis, accumulation of propionyl-CoA, morphology of conidia, deletion leads to an attenuation of virulence, overview
Aspergillus fumigatus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetyl-CoA
competitive to CoA
Aspergillus fumigatus
propionyl-CoA
competitive to CoA
Aspergillus fumigatus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.053
-
propionyl-CoA
versus CoA
Aspergillus fumigatus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0019
-
propanoyl-CoA
pH 8.0, 25°C, purified native enzyme
Aspergillus fumigatus
0.0026
-
acetyl-CoA
pH 8.0, 25°C, purified native enzyme
Aspergillus fumigatus
0.0027
-
oxaloacetate
pH 8.0, 25°C, purified native enzyme
Aspergillus fumigatus
0.0085
-
CoA
pH 8.0, 25°C, purified native enzyme, in absence of propionyl-CoA
Aspergillus fumigatus
0.0325
-
CoA
pH 8.0, 25°C, purified native enzyme, in presence of propionyl-CoA
Aspergillus fumigatus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
enzyme possesses a mitochondrial signal import sequence
Aspergillus fumigatus
5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
x * 45000, native enzyme, SDS-PAGE, x * 514100, sequence calculation
Aspergillus fumigatus
514100
-
x * 45000, native enzyme, SDS-PAGE, x * 514100, sequence calculation
Aspergillus fumigatus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Aspergillus fumigatus
key enzyme of the methylcitrate cycle and required for fungal propionate degradation
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme 136fold to homogeneity
Aspergillus fumigatus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus fumigatus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
17.7
-
purified native enzyme, with oxaloacetate and propionyl-CoA
Aspergillus fumigatus
48
-
purified native enzyme, with oxaloacetate and acetyl-CoA
Aspergillus fumigatus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + H2O + oxaloacetate
the enzyme also performs the citrate synthase reaction, EC 2.3.3.1
661700
Aspergillus fumigatus
citrate + CoA
-
-
-
?
additional information
key enzyme of the methylcitrate cycle and required for fungal propionate degradation
661700
Aspergillus fumigatus
?
-
-
-
-
propanoyl-CoA + H2O + oxaloacetate
reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate
661700
Aspergillus fumigatus
(2S,3S)-2-methylcitrate + CoA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 45000, native enzyme, SDS-PAGE, x * 514100, sequence calculation
Aspergillus fumigatus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
60
-
Aspergillus fumigatus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
3 h, stable up to
Aspergillus fumigatus
60
-
half-life 11 min
Aspergillus fumigatus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Aspergillus fumigatus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
9
3 h, stable
Aspergillus fumigatus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Aspergillus fumigatus
2 peaks
-
6.9
Aspergillus fumigatus
2 peaks
-
9
Other publictions for EC 2.3.3.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735578
Eniyan
Cloning, expression, purificat ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Asian Pac. J. Trop. Med.
8
19-23
2015
-
-
1
-
-
-
-
-
1
-
2
2
-
161
-
-
1
-
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-
-
-
2
1
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-
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-
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1
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1
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1
-
2
2
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1
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-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
735795
Kobayashi
Gene identification and functi ...
Aspergillus niger, Aspergillus niger WU-2223L
Biosci. Biotechnol. Biochem.
77
1492-1498
2013
-
-
1
-
-
-
-
-
1
-
1
2
-
5
-
-
1
-
-
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4
1
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1
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1
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1
2
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1
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-
4
1
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-
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-
-
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-
-
-
1
1
-
-
-
720336
Chittori
Crystal structure of Salmonell ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Struct. Biol.
174
58-68
2011
-
-
-
-
-
-
2
4
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4
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3
1
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1
2
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2
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4
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-
3
1
-
-
1
2
-
-
-
-
-
-
-
-
2
2
704332
Rocco
In Salmonella enterica, 2-meth ...
Salmonella enterica, Salmonella enterica JE2170
J. Bacteriol.
192
771-778
2010
-
-
-
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
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-
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-
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1
-
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-
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-
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
705576
Domin
Methylcitrate cycle activation ...
Fusarium solani, Fusarium verticillioides
Microbiology
155
3903-3912
2009
-
-
2
-
-
-
-
8
-
-
2
2
-
9
-
-
2
-
-
-
4
-
4
-
-
2
-
4
-
2
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
8
-
-
2
2
-
-
-
2
-
-
4
-
4
-
-
2
-
4
-
2
-
-
-
2
2
-
-
-
703115
Ibrahim-Granet
Methylcitrate synthase from As ...
Aspergillus fumigatus
Cell. Microbiol.
10
134-148
2008
-
1
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
6
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
6
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
705770
Fleck
Characterization of an acyl-Co ...
Aspergillus nidulans
Mol. Microbiol.
68
642-656
2008
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
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-
-
-
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1
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1
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1
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1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
671529
Ewering
Occurrence and expression of t ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Appl. Microbiol. Biotechnol.
71
80-89
2006
-
-
1
-
-
-
-
2
-
-
1
-
-
5
-
-
-
-
-
-
-
-
8
1
1
-
-
-
1
-
-
-
-
1
-
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-
1
-
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-
-
-
-
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2
-
-
1
-
-
-
-
-
-
-
-
-
8
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
675795
Ewering
Metabolic engineering of strai ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Metab. Eng.
8
587-602
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
46
-
-
-
-
-
-
-
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1
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-
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-
-
-
-
-
-
-
-
-
-
-
661700
Maerker
Methylcitrate synthase from As ...
Aspergillus fumigatus
FEBS J.
272
3615-3630
2005
-
-
-
-
1
-
2
5
1
-
2
1
-
5
-
-
1
-
-
2
2
-
3
1
1
-
2
-
1
-
1
-
1
2
-
-
-
-
-
-
1
-
-
2
1
5
1
-
2
1
-
-
-
1
-
2
2
-
3
1
1
-
2
-
1
-
1
2
-
-
-
-
-
-
488087
Karlsson
Rhodothermus marinus: a thermo ...
Escherichia coli, Pseudomonas aeruginosa, Rhodothermus marinus
Extremophiles
6
51-56
2002
-
-
2
-
-
-
-
4
-
-
-
3
-
3
-
-
1
-
-
-
1
-
8
2
1
1
1
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-
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2
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-
-
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4
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3
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1
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-
1
-
8
2
1
1
1
-
-
-
-
-
-
-
-
-
-
-
488174
Braemer
Identification of the 2-methyl ...
Aspergillus nidulans, Burkholderia cepacia, Burkholderia cepacia contig261, Cupriavidus metallidurans, Escherichia coli, Escherichia coli O157:H17, Legionella pneumophila, Neisseria meningitidis, Paraburkholderia sacchari, Paraburkholderia sacchari IPT101, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas fluorescens contig267, Pseudomonas putida, Pseudomonas putida contig10768, Pseudomonas putida KT 2240, Pseudomonas syringae, Pseudomonas syringae contig5376, Pyrococcus furiosus, Salmonella enterica, Shewanella putrefaciens, Shewanella putrefaciens contig93, Vibrio cholerae
Appl. Environ. Microbiol.
68
271-279
2002
-
-
1
-
-
-
-
-
-
-
1
33
-
74
-
-
-
-
-
-
-
-
41
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
33
-
-
-
-
-
-
-
-
41
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488175
Brock
Oxidation of propionate to pyr ...
Cupriavidus metallidurans, Escherichia coli, Salmonella enterica, Yarrowia lipolytica
Eur. J. Biochem.
269
6184-6194
2002
-
-
2
-
-
-
-
-
-
-
1
5
-
7
-
-
1
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
5
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488176
Claes
Identification of two prpDBC g ...
Aspergillus nidulans, Corynebacterium glutamicum, Cupriavidus metallidurans, Escherichia coli, Paraburkholderia sacchari, Pseudomonas aeruginosa, Salmonella enterica, Yarrowia lipolytica
J. Bacteriol.
184
2728-2739
2002
-
-
1
-
-
-
-
-
-
-
2
12
-
15
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
12
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
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488173
Braemer
The methylcitric acid pathway ...
Aspergillus nidulans, Cupriavidus metallidurans, Escherichia coli, Neisseria meningitidis, Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas putida KT 2240, Salmonella enterica, Vibrio cholerae
Microbiology
147
2203-2214
2001
-
1
1
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1
9
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49
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13
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1
1
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1
9
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13
-
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488089
Brock
Methylcitrate synthase from As ...
Aspergillus nidulans
Mol. Microbiol.
35
961-973
2000
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-
1
-
1
-
2
3
2
1
3
1
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3
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1
1
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1
4
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3
1
1
1
-
2
1
1
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-
-
-
-
-
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1
-
-
1
-
-
2
-
3
2
1
3
1
-
-
1
1
-
1
4
-
3
1
1
1
-
2
1
1
-
-
-
-
-
-
-
-
488172
Gerike
-
Citrate synthase and 2-methylc ...
Arthrobacter sp., Escherichia coli, Escherichia coli JM109, no activity in Sus scrofa, Pyrococcus furiosus, Saccharomyces cerevisiae, Salmonella enterica, Thermoplasma acidophilum, Yarrowia lipolytica
Microbiology
144
929-935
1998
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1
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5
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8
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38
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3
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10
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1
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5
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8
-
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3
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-
-
10
-
-
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-
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488171
Textor
Propionate oxidation in Escher ...
Escherichia coli, Saccharomyces cerevisiae, Salmonella enterica, Yarrowia lipolytica
Arch. Microbiol.
168
428-436
1997
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-
-
-
-
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1
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1
6
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10
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1
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1
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6
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1
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1
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1
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1
6
-
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1
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1
-
6
-
1
-
-
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1
-
-
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-
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-
-
-
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488170
Pronk
-
Propionate metabolism in Sacch ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae CBS 8066, Yarrowia lipolytica
Microbiology
140
717-722
1994
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-
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3
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4
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5
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3
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5
-
-
-
-
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-
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-
-
-
5348
Miyakoshi
-
Distribution of the methylcitr ...
Aspergillus niger, Candida gropengiesseri, Citeromyces matritensis, Cryptococcus neoformans, Cutaneotrichosporon curvatus, Cyberlindnera saturnus, Cyberlindnera saturnus IAM 4094, Debaryomyces hansenii, Diutina catenulata, Kluyveromyces marxianus, Lipomyces starkeyi, Metschnikowia pulcherrima, Mucor rouxianus, Neurospora crassa, no activity in Candida rugosa, Ogataea angusta, Priceomyces haplophilus, Pseudomonas aeruginosa, Rhizopus microsporus var. chinensis, Rhizopus microsporus var. chinensis IAM 6003, Saccharomyces cerevisiae, Saccharomyces cerevisiae SANK 50182, Saccharomycopsis fibuligera, Schwanniomyces polymorphus, Starmerella magnoliae, Torulaspora delbrueckii, Ustilago crus-galli, Ustilago crus-galli F-B-6, Ustilago utriculosa, Ustilago utriculosa F-B-5, Vanrija humicola, Wickerhamiella pararugosa, Wickerhamomyces anomalus, Yarrowia lipolytica
Agric. Biol. Chem.
51
2381-2387
1987
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36
-
34
-
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36
-
43
-
-
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-
-
-
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36
-
-
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36
-
43
-
-
-
-
-
-
-
-
-
-
-
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-
-
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488168
Watson
-
Pseudomonas aeruginosa contain ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa PAO, Pseudomonas citronellolis, Pseudomonas putida, Yarrowia lipolytica
Curr. Microbiol.
8
17-21
1983
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-
-
-
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-
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5
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11
-
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1
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14
-
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5
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1
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14
-
-
-
-
-
-
-
-
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-
-
-
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5344
Uchiyama
Subcellular localization of th ...
Yarrowia lipolytica
Eur. J. Biochem.
125
523-527
1982
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-
-
-
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1
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1
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1
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4
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3
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1
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1
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4
-
3
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488166
Uchiyama
-
Properties of methylcitrate sy ...
Escherichia coli, Yarrowia lipolytica
Agric. Biol. Chem.
40
1411-1418
1976
1
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9
5
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3
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3
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2
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1
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2
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9
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1
1
1
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1
1
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1
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-
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9
-
5
-
3
-
3
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1
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2
-
9
-
1
1
1
-
1
1
-
-
-
-
-
-
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488165
Tabuchi
-
Methylcitrate condensing and m ...
Yarrowia lipolytica
Agric. Biol. Chem.
39
2035-2042
1975
-
-
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1
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1
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1
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5
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1
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1
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1
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5
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1
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