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Literature summary for 2.3.3.5 extracted from

  • Uchiyama, H.; Tabuchi, T.
    Properties of methylcitrate synthase from Candida lipolytica (1976), Agric. Biol. Chem., 40, 1411-1418.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
isocitrate 1 mM, relative activity 101% Yarrowia lipolytica

Inhibitors

Inhibitors Comment Organism Structure
ADP slight inhibition Yarrowia lipolytica
ATP
-
Yarrowia lipolytica
H2O2
-
Yarrowia lipolytica
Hg2+
-
Yarrowia lipolytica
Monoiodoacetamide
-
Yarrowia lipolytica
NADH
-
Yarrowia lipolytica
NADPH
-
Yarrowia lipolytica
p-chloromercuribenzoate
-
Yarrowia lipolytica
Zn2+
-
Yarrowia lipolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0029
-
acetyl-CoA
-
Yarrowia lipolytica
0.0053
-
propionyl-CoA
-
Yarrowia lipolytica
0.022
-
butyryl-CoA
-
Yarrowia lipolytica
0.025
-
oxaloacetate
-
Yarrowia lipolytica
0.025
-
valeryl-CoA
-
Yarrowia lipolytica

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, relative activity 104% Yarrowia lipolytica
Fe2+ 0.02 mM, relative activity 104% Yarrowia lipolytica
Mg2+ 1 mM, relative activity 102% Yarrowia lipolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
propanoyl-CoA + H2O + oxaloacetate Yarrowia lipolytica physiological role of the methylcitric acid cycle is a catabolic reaction sequence concerning the oxidation of propionate into pyruvate, regulatory enzyme of this cycle 2-methylcitrate + CoA
-
?
propanoyl-CoA + H2O + oxaloacetate Escherichia coli first key enzyme of the methylcitric acid cycle 2-methylcitrate + CoA
-
?
propanoyl-CoA + H2O + oxaloacetate Yarrowia lipolytica first key enzyme of the methylcitric acid cycle 2-methylcitrate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Yarrowia lipolytica
-
formerly Candida lipolytica
-
Yarrowia lipolytica
-
IFO 1659
-

Purification (Commentary)

Purification (Comment) Organism
-
Yarrowia lipolytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.436
-
substrate propionyl-CoA Yarrowia lipolytica
0.623
-
substrate acetyl-CoA Yarrowia lipolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate
-
Yarrowia lipolytica citrate + CoA
-
?
additional information enzyme is specific for oxaloacetate, does not catalyze liberation of CoA from acetyl- or propionyl-CoA in presence of glyoxylate, pyruvate or alpha-ketoglutarate, isobutyryl-CoA and isovaleryl-CoA are no substrates Yarrowia lipolytica ?
-
?
n-butyryl-CoA + H2O + oxaloacetate
-
Yarrowia lipolytica 2-ethylcitrate + CoA
-
r
n-valeryl-CoA + H2O + oxaloacetate
-
Yarrowia lipolytica 2-propylcitrate + CoA
-
r
propanoyl-CoA + H2O + oxaloacetate
-
Escherichia coli 2-methylcitrate + CoA
-
?
propanoyl-CoA + H2O + oxaloacetate
-
Yarrowia lipolytica 2-methylcitrate + CoA
-
?
propanoyl-CoA + H2O + oxaloacetate physiological role of the methylcitric acid cycle is a catabolic reaction sequence concerning the oxidation of propionate into pyruvate, regulatory enzyme of this cycle Yarrowia lipolytica 2-methylcitrate + CoA
-
?
propanoyl-CoA + H2O + oxaloacetate first key enzyme of the methylcitric acid cycle Escherichia coli 2-methylcitrate + CoA
-
?
propanoyl-CoA + H2O + oxaloacetate first key enzyme of the methylcitric acid cycle Yarrowia lipolytica 2-methylcitrate + CoA
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Yarrowia lipolytica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 50
-
Yarrowia lipolytica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
activity completely lost after 10 min Yarrowia lipolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
Yarrowia lipolytica

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5
-
Yarrowia lipolytica