Cloned (Comment) | Organism |
---|---|
gene prpC sequenced | Escherichia coli |
gene sequenced | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
oxaloacetate | second substrate propionyl-CoA | Rhodothermus marinus | |
0.003 | - |
oxaloacetate | - |
Rhodothermus marinus | |
0.003 | - |
propionyl-CoA | - |
Rhodothermus marinus | |
0.007 | - |
acetyl-CoA | - |
Rhodothermus marinus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
propanoyl-CoA + H2O + oxaloacetate | Pseudomonas aeruginosa | - |
2-methylcitrate + CoA | - |
? | |
propanoyl-CoA + H2O + oxaloacetate | Rhodothermus marinus | - |
2-methylcitrate + CoA | - |
? | |
propanoyl-CoA + H2O + oxaloacetate | Escherichia coli | implicated in the metabolism of propionate | 2-methylcitrate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Pseudomonas aeruginosa | - |
- |
- |
Rhodothermus marinus | - |
R-10 (DSM 4252) | - |
Purification (Comment) | Organism |
---|---|
- |
Rhodothermus marinus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28 | - |
- |
Rhodothermus marinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | - |
Rhodothermus marinus | citrate + CoA | - |
? | |
additional information | constitutive | Rhodothermus marinus | ? | - |
? | |
additional information | enzyme is induced by growth on propionate, propanol and heptanoate | Escherichia coli | ? | - |
? | |
additional information | enzyme is induced by growth on propionate, propanol and heptanoate | Pseudomonas aeruginosa | ? | - |
? | |
propanoyl-CoA + H2O + oxaloacetate | - |
Escherichia coli | 2-methylcitrate + CoA | - |
? | |
propanoyl-CoA + H2O + oxaloacetate | - |
Pseudomonas aeruginosa | 2-methylcitrate + CoA | - |
? | |
propanoyl-CoA + H2O + oxaloacetate | - |
Rhodothermus marinus | 2-methylcitrate + CoA | - |
? | |
propanoyl-CoA + H2O + oxaloacetate | implicated in the metabolism of propionate | Escherichia coli | 2-methylcitrate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Escherichia coli |
dimer | - |
Rhodothermus marinus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Rhodothermus marinus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | - |
Rhodothermus marinus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
half-life at 90°C is 12 min, active even at mesophilic temperatures, at 40°C enzyme retains 20-30% of the maximum activity | Rhodothermus marinus |