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Literature summary for 2.3.3.5 extracted from

  • Karlsson, E.N.; Abou-Hachem, M.; Holst, O.; Danson, M.J.; Hough, D.W.
    Rhodothermus marinus: a thermophilic bacterium producing dimeric and hexameric citrate synthase isoenzymes (2002), Extremophiles, 6, 51-56.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene prpC sequenced Escherichia coli
gene sequenced Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
oxaloacetate second substrate propionyl-CoA Rhodothermus marinus
0.003
-
oxaloacetate
-
Rhodothermus marinus
0.003
-
propionyl-CoA
-
Rhodothermus marinus
0.007
-
acetyl-CoA
-
Rhodothermus marinus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
propanoyl-CoA + H2O + oxaloacetate Pseudomonas aeruginosa
-
2-methylcitrate + CoA
-
?
propanoyl-CoA + H2O + oxaloacetate Rhodothermus marinus
-
2-methylcitrate + CoA
-
?
propanoyl-CoA + H2O + oxaloacetate Escherichia coli implicated in the metabolism of propionate 2-methylcitrate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Pseudomonas aeruginosa
-
-
-
Rhodothermus marinus
-
R-10 (DSM 4252)
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodothermus marinus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28
-
-
Rhodothermus marinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate
-
Rhodothermus marinus citrate + CoA
-
?
additional information constitutive Rhodothermus marinus ?
-
?
additional information enzyme is induced by growth on propionate, propanol and heptanoate Escherichia coli ?
-
?
additional information enzyme is induced by growth on propionate, propanol and heptanoate Pseudomonas aeruginosa ?
-
?
propanoyl-CoA + H2O + oxaloacetate
-
Escherichia coli 2-methylcitrate + CoA
-
?
propanoyl-CoA + H2O + oxaloacetate
-
Pseudomonas aeruginosa 2-methylcitrate + CoA
-
?
propanoyl-CoA + H2O + oxaloacetate
-
Rhodothermus marinus 2-methylcitrate + CoA
-
?
propanoyl-CoA + H2O + oxaloacetate implicated in the metabolism of propionate Escherichia coli 2-methylcitrate + CoA
-
?

Subunits

Subunits Comment Organism
dimer
-
Escherichia coli
dimer
-
Rhodothermus marinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Rhodothermus marinus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 90
-
Rhodothermus marinus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
half-life at 90°C is 12 min, active even at mesophilic temperatures, at 40°C enzyme retains 20-30% of the maximum activity Rhodothermus marinus