BRENDA - Enzyme Database show
show all sequences of 2.3.3.3

Reversal of the stereospecificity of the citrate synthase of Clostridium kluyveri by p-chloromercuribenzoate

O'Brien, R.W.; Stern, J.R.; Biochem. Biophys. Res. Commun. 34, 271-276 (1969)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
4-chloromercuribenzoate
1 mM and 5 mM, 35 and 42% inhibition in extracts of Barker strain, respectively
Clostridium kluyveri
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium kluyveri
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + H2O + oxaloacetate
stereospecificity of citrate synthase in extracts can be changed reversibly from the (R)-type to the (S)-type by treatment with 4-chloromercuribenzoate
488161
Clostridium kluyveri
citrate + CoA
-
488161
Clostridium kluyveri
-
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
4-chloromercuribenzoate
1 mM and 5 mM, 35 and 42% inhibition in extracts of Barker strain, respectively
Clostridium kluyveri
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + H2O + oxaloacetate
stereospecificity of citrate synthase in extracts can be changed reversibly from the (R)-type to the (S)-type by treatment with 4-chloromercuribenzoate
488161
Clostridium kluyveri
citrate + CoA
-
488161
Clostridium kluyveri
-
Other publictions for EC 2.3.3.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735509
Kim
Two pathways for glutamate bio ...
Syntrophus aciditrophicus
Appl. Environ. Microbiol.
81
8434-8444
2015
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
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-
1
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-
-
-
-
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-
-
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1
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-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
736357
Kim
Identification and characteriz ...
Syntrophus aciditrophicus, Syntrophus aciditrophicus ATCC 700169
J. Bacteriol.
195
1689-1696
2013
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-
1
-
-
-
7
2
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5
4
2
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5
-
-
1
-
-
1
2
1
4
1
-
-
1
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-
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-
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-
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-
1
-
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-
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7
-
2
-
5
4
2
-
-
-
1
-
1
2
1
4
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
719729
Marco-Urrea
Identification and characteriz ...
Dehalococcoides sp., Dehalococcoides sp. CBDB1
J. Bacteriol.
193
5171-5178
2011
1
-
1
-
-
-
4
-
-
1
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4
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1
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4
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1
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1
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-
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4
-
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1
-
-
-
-
-
1
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4
-
-
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-
-
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-
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-
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-
-
-
674333
Li
Re-citrate synthase from Clost ...
Clostridium kluyveri, Desulfovibrio desulfuricans, Desulfovibrio vulgaris
J. Bacteriol.
189
4299-4304
2007
-
-
1
-
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-
3
2
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-
2
-
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5
-
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1
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1
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1
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1
1
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-
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1
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-
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3
-
2
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2
-
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-
1
1
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1
-
1
1
-
-
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-
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488163
Gottschalk
re-Citrate synthase reaction ...
Clostridium acidi-urici
Eur. J. Biochem.
26
455-461
1972
-
-
-
-
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-
2
-
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1
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-
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-
3
-
-
-
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-
1
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-
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2
-
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-
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-
-
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-
-
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-
3
-
-
-
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-
1
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-
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-
-
-
-
-
488162
Wunderwald
Stereochemistry of the re-citr ...
Clostridium acidi-urici
Eur. J. Biochem.
24
216-221
1971
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1
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1
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1
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-
488158
Goschalk
-
Properties of (R)-citrate synt ...
Clostridium acidi-urici
Hoppe-Seyler's Z. Physiol. chem.
351
1183-1190
1970
1
-
-
-
-
-
8
2
-
2
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-
1
-
-
1
-
-
-
1
-
1
-
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-
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1
-
-
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-
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-
-
8
-
2
-
2
-
-
-
-
-
1
-
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1
-
1
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-
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-
-
-
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-
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-
-
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-
-
488157
Gottschalk
Partial purification and some ...
Clostridium acidi-urici
Eur. J. Biochem.
7
301-106
1969
3
-
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-
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1
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2
1
-
1
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1
1
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1
1
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3
-
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1
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1
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1
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1
1
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1
1
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-
-
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-
488159
Dittbrenner
The stereospecificity of the ( ...
Clostridium acidi-urici, Clostridium kluyveri
Biochem. Biophys. Res. Commun.
36
802-808
1969
-
-
-
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2
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2
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1
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2
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2
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1
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2
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488160
Stern
Failure of ethylenediaminetetr ...
Clostridium cylindrosporum, Clostridium kluyveri
FEBS Lett.
4
72-74
1969
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2
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2
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2
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2
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2
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2
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2
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488161
O'Brien
Reversal of the stereospecific ...
Clostridium kluyveri
Biochem. Biophys. Res. Commun.
34
271-276
1969
-
-
-
-
-
-
1
-
-
-
-
-
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1
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1
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1
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1
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