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Literature summary for 2.3.3.21 extracted from
- Eliminating acetate formation improves citramalate production by metabolically engineered Escherichia coli (2017), Microb. Cell Fact., 16, 114 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | synthesis of citramalate at high yield by Escherichia coli overexpressing citramalate synthase. Citramalate is a chemical precursor to the industrially important methacrylic acid. Acetate is an undesirable by-product potentially formed from pyruvate and acetyl-CoA, the precursors of citramalate during aerobic growth of Escherichia coli. Gene deletions critical to reducing acetate accumulation during aerobic growth and citramalate production are ifdentified in metabolically engineered Escherichia coli strains. The key knockouts critical to minimizing acetate formation are identified as pta, ackA and poxB | Methanocaldococcus jannaschii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli MG1655 cells | Escherichia coli |
| expression in Escherichia coli | Methanocaldococcus jannaschii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + pyruvate + H2O | Escherichia coli | - |
CoA + citramalate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Methanocaldococcus jannaschii | Q58787 | - |
- |
| Methanocaldococcus jannaschii DSM 2661 | Q58787 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetate + pyruvate | - |
Methanocaldococcus jannaschii | citramalate | - |
? | |
| acetate + pyruvate | - |
Methanocaldococcus jannaschii DSM 2661 | citramalate | - |
? | |
| acetyl-CoA + pyruvate + H2O | - |
Escherichia coli | CoA + citramalate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CimA | - |
Methanocaldococcus jannaschii |
| CimA | - |
Escherichia coli |
| citramalate synthase | - |
Escherichia coli |
| citramalate synthase | - |
Methanocaldococcus jannaschii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | key enzyme in metabolic pathways for citramalate synthesis | Methanocaldococcus jannaschii |
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Release 2023.1 (January 2023)
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