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show all sequences of 2.3.3.18

Studies on the biosynthesis of bialaphos (SF-1293). 8. Purification and characterization of 2-phosphinomethylmalic acid synthase from Streptomyces hygroscopicus SF-1293

Shimotohno, K.; Seto, H.; Ōtake, N.; Imai, S.; Murakami, T.; J. Antibiot. 41, 1057-1065 (1988)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
4-chloromercuribenzoate
0.1 mM, 2% residual activity
Streptomyces hygroscopicus
Ca2+
1 mM, 54% residual activity
Streptomyces hygroscopicus
EDTA
0.05 mM, almost complete inactivation
Streptomyces hygroscopicus
Fe2+
1 mM, 50% residual activity
Streptomyces hygroscopicus
iodoacetic acid
1 mM, 18% residual activity
Streptomyces hygroscopicus
Mg2+
1 mM, 44% residual activity
Streptomyces hygroscopicus
phosphate buffer
not inhibitory at 10 mM, 50% loss of activity in 100 mM phosphate buffer
Streptomyces hygroscopicus
Zn2+
1 mM, 24% residual activity
Streptomyces hygroscopicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.052
-
acetyl-CoA
pH 8.0, 30C
Streptomyces hygroscopicus
0.13
-
oxaloacetic acid
pH 8.0, 30C
Streptomyces hygroscopicus
0.385
-
3-(hydroxyphosphinoyl)pyruvate
pH 8.0, 30C
Streptomyces hygroscopicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
2 * 48000, SDS-PAGE
Streptomyces hygroscopicus
90000
98000
gel fitlration
Streptomyces hygroscopicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
Streptomyces hygroscopicus
-
phosphinomethylmalate + CoA
-
-
?
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
Streptomyces hygroscopicus SF-1293
-
phosphinomethylmalate + CoA
-
-
?
acetyl-CoA + H2O + oxaloacetic acid
Streptomyces hygroscopicus
-
? + CoA
-
-
?
acetyl-CoA + H2O + oxaloacetic acid
Streptomyces hygroscopicus SF-1293
-
? + CoA
-
-
?
additional information
Streptomyces hygroscopicus
no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate
?
-
-
-
additional information
Streptomyces hygroscopicus SF-1293
no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces hygroscopicus
Q9LCB4
-
-
Streptomyces hygroscopicus SF-1293
Q9LCB4
-
-
Purification (Commentary)
Commentary
Organism
-
Streptomyces hygroscopicus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
546.8
-
pH 8.0, 30C
Streptomyces hygroscopicus
Storage Stability
Storage Stability
Organism
4C, lyophilized powder, 5% loss of activity within 3 months
Streptomyces hygroscopicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
-
740585
Streptomyces hygroscopicus
phosphinomethylmalate + CoA
-
-
-
?
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
-
740585
Streptomyces hygroscopicus SF-1293
phosphinomethylmalate + CoA
-
-
-
?
acetyl-CoA + H2O + oxaloacetic acid
-
740585
Streptomyces hygroscopicus
? + CoA
-
-
-
?
acetyl-CoA + H2O + oxaloacetic acid
-
740585
Streptomyces hygroscopicus SF-1293
? + CoA
-
-
-
?
additional information
no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate
740585
Streptomyces hygroscopicus
?
-
-
-
-
additional information
no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate
740585
Streptomyces hygroscopicus SF-1293
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 48000, SDS-PAGE
Streptomyces hygroscopicus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Streptomyces hygroscopicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Streptomyces hygroscopicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
4-chloromercuribenzoate
0.1 mM, 2% residual activity
Streptomyces hygroscopicus
Ca2+
1 mM, 54% residual activity
Streptomyces hygroscopicus
EDTA
0.05 mM, almost complete inactivation
Streptomyces hygroscopicus
Fe2+
1 mM, 50% residual activity
Streptomyces hygroscopicus
iodoacetic acid
1 mM, 18% residual activity
Streptomyces hygroscopicus
Mg2+
1 mM, 44% residual activity
Streptomyces hygroscopicus
phosphate buffer
not inhibitory at 10 mM, 50% loss of activity in 100 mM phosphate buffer
Streptomyces hygroscopicus
Zn2+
1 mM, 24% residual activity
Streptomyces hygroscopicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.052
-
acetyl-CoA
pH 8.0, 30C
Streptomyces hygroscopicus
0.13
-
oxaloacetic acid
pH 8.0, 30C
Streptomyces hygroscopicus
0.385
-
3-(hydroxyphosphinoyl)pyruvate
pH 8.0, 30C
Streptomyces hygroscopicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
2 * 48000, SDS-PAGE
Streptomyces hygroscopicus
90000
98000
gel fitlration
Streptomyces hygroscopicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
Streptomyces hygroscopicus
-
phosphinomethylmalate + CoA
-
-
?
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
Streptomyces hygroscopicus SF-1293
-
phosphinomethylmalate + CoA
-
-
?
acetyl-CoA + H2O + oxaloacetic acid
Streptomyces hygroscopicus
-
? + CoA
-
-
?
acetyl-CoA + H2O + oxaloacetic acid
Streptomyces hygroscopicus SF-1293
-
? + CoA
-
-
?
additional information
Streptomyces hygroscopicus
no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate
?
-
-
-
additional information
Streptomyces hygroscopicus SF-1293
no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces hygroscopicus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
546.8
-
pH 8.0, 30C
Streptomyces hygroscopicus
Storage Stability (protein specific)
Storage Stability
Organism
4C, lyophilized powder, 5% loss of activity within 3 months
Streptomyces hygroscopicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
-
740585
Streptomyces hygroscopicus
phosphinomethylmalate + CoA
-
-
-
?
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
-
740585
Streptomyces hygroscopicus SF-1293
phosphinomethylmalate + CoA
-
-
-
?
acetyl-CoA + H2O + oxaloacetic acid
-
740585
Streptomyces hygroscopicus
? + CoA
-
-
-
?
acetyl-CoA + H2O + oxaloacetic acid
-
740585
Streptomyces hygroscopicus SF-1293
? + CoA
-
-
-
?
additional information
no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate
740585
Streptomyces hygroscopicus
?
-
-
-
-
additional information
no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate
740585
Streptomyces hygroscopicus SF-1293
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 48000, SDS-PAGE
Streptomyces hygroscopicus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Streptomyces hygroscopicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Streptomyces hygroscopicus
Other publictions for EC 2.3.3.18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739833
Shimotohno
Purification and characterizat ...
Streptomyces hygroscopicus, Streptomyces hygroscopicus SF-1293
Agric. Biol. Chem.
54
463-470
1990
2
-
-
-
-
-
5
2
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
5
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740585
Shimotohno
Studies on the biosynthesis of ...
Streptomyces hygroscopicus, Streptomyces hygroscopicus SF-1293
J. Antibiot.
41
1057-1065
1988
-
-
-
-
-
-
8
3
-
-
2
6
-
8
-
-
1
-
-
-
1
1
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
3
-
-
2
6
-
-
-
1
-
-
1
1
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-